Harayama H, Miyano T, Miyake M, Kusunoki H, Kato S
Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe-shi, Japan.
Mol Reprod Dev. 1994 Apr;37(4):436-45. doi: 10.1002/mrd.1080370410.
The present report identifies epididymal boar anti-agglutinin and examines its effect on sperm motility. Boar spermatozoa from the cauda epididymidis were washed and incubated in modified Krebs-Ringer bicarbonate at 37 degrees C (5% CO2 in air). In the samples washed three or five times and then incubated for 3-5 h, higher rates (72-79%) of spermatozoa were associated with one another at the acrosomal region, mainly in groups of 2-5 cells (head-to-head agglutination), and many cells exhibited intensively flagellant and/or circular types of movement but rarely progressive motility. The addition of epididymal plasma or 25 kDa protein purified from it markedly inhibited the occurrence of head-to-head agglutination in washed spermatozoa, whereas heat treatment and subsequent removal of insoluble materials reduced the anti-agglutination activity of epididymal plasma. The percentages of progressively motile cells in the samples incubated with epididymal plasma or 25 kDa epididymal protein rose coincident with the reduction of sperm agglutination. These findings demonstrate that the 25 kDa epididymal protein is an anti-agglutinin for the cauda spermatozoa and that it effectively functions to maintain progressive motility of the cells in vitro.
本报告鉴定了附睾公猪抗凝集素，并研究了其对精子活力的影响。从附睾尾部采集的公猪精子经洗涤后，在改良的 Krebs-Ringer 碳酸氢盐溶液中于 37℃（空气中 5%二氧化碳）孵育。在洗涤 3 次或 5 次后再孵育 3 - 5 小时的样本中，较高比例（72 - 79%）的精子在顶体区域相互聚集，主要为 2 - 5 个细胞的群体（头对头凝集），许多细胞表现出强烈的鞭毛运动和/或圆周运动类型，但很少有前进运动。添加附睾血浆或从中纯化的 25 kDa 蛋白可显著抑制洗涤后精子头对头凝集的发生，而热处理及随后去除不溶性物质则降低了附睾血浆的抗凝集活性。与附睾血浆或 25 kDa 附睾蛋白孵育的样本中，前进运动细胞的百分比随着精子凝集的减少而升高。这些发现表明，25 kDa 附睾蛋白是附睾尾部精子的抗凝集素，并且它在体外能有效地维持细胞的前进运动。
