[肾癌中的Wnt/Frizzled信号通路]
[Wnt/Frizzled signaling pathway in renal carcinoma].
作者信息
Gong Kan, Lin Gui-ting, Zhang Zhi-wen, Chang Zhi-jie, Na Yan-qun
机构信息
Institute of Urology, Department of Urology, First Hospital of Peking University. Beijing 100034, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2003 Jul 25;83(14):1270-3.
OBJECTIVE
To investigate the transduction of Wnt/Frizzled signaling pathway, especially the function of T cell factor 4 (TCF(4)), in renal carcinoma.
METHODS
A renal carcinoma yeast two hybrid library and a human TCF(4) yeast two hybrid expression vector were constructed. Proteins interacting with the bait protein human TCF(4) were obtained from the renal carcinoma yeast two hybrid library by reverse yeast two hybrid system.
RESULTS
67 positive clones interacting with the bait protein TCF(4) were obtained by reverse yeast two hybrid system, including 18 beta-catenin clones, 24 TCF(4) clones and 25 unknown clones.
CONCLUSION
Wnt/Frizzled signaling pathway exists in renal carcinoma. TCF(4), its important signal factor, interacts with beta-catenin and forms homodimer or homocopolymer by itself, thus displaying its constitutive transcriptional activity.
目的
研究Wnt/Frizzled信号通路在肾癌中的转导情况，尤其是T细胞因子4（TCF(4)）的功能。
方法
构建肾癌酵母双杂交文库及人TCF(4)酵母双杂交表达载体。通过反向酵母双杂交系统从肾癌酵母双杂交文库中获取与诱饵蛋白人TCF(4)相互作用的蛋白质。
结果
通过反向酵母双杂交系统获得67个与诱饵蛋白TCF(4)相互作用的阳性克隆，其中包括18个β-连环蛋白克隆、24个TCF(4)克隆和25个未知克隆。
结论
Wnt/Frizzled信号通路存在于肾癌中。其重要信号因子TCF(4)与β-连环蛋白相互作用，并自身形成同源二聚体或同源多聚体，从而发挥其组成型转录活性。
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