Liu Bing-ci, Xu Zeng-lu, Miao Qing, Xu Yuan-yuan, Xu Ming, Qian Xiao-jing, You Bao-rong, Yuan Bo-hua, Kang Ning
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Jul;37(4):243-5.
To investigate the effects of excessive intake of fluoride on the expression of type II collagen gene and types and morphological change of collagen fiber in the bone tissues of rats.
A rat model with fluorosis was established by adding 221 mg/L of sodium fluoride (NaF) to drinking water for the rats for 15 days, 30 days and two months, respectively. Type II collagen alpha1 (II) cDNA probe was prepared, and cDNA-mRNA in-situ hybridization was employed to detect change in expression of type II collagen mRNA in the bone tissues of rats with excessive intake of fluoride (221 mg/L NaF). Picrosirius-polarization method was used to observe types of collagen and morphology of collagen fiber in the bone tissues.
Chondroblasts were found in the femur and other bone tissues of the rats after exposure to fluoride. cDNA-mRNA in-situ hybridization showed that expression of type II collagen gene could be observed in the cytoplasm of chondrocytic lacuna and chondrified bone tissues. mRNA in collagen of chondrocytes of the rib cartilage reached the peak level 15 days after exposure to fluoride, and decreased gradually one month and two months after exposure. Polychromatic type II collagen, breakage of collagen fiber, disorder array and reduced content of type II collagen could be found in the bone tissues with picrosirius-polarization method.
Excessive intake of fluoride could lead to changes in types and structure of collagen (cross-linkage) of bone tissues, which caused expression of type II collagen gene in the chondrified bone tissues and enhanced its expression in the rib cartilage tissues.
探讨过量摄入氟对大鼠骨组织Ⅱ型胶原基因表达及胶原纤维类型和形态变化的影响。
分别给大鼠饮用含221mg/L氟化钠（NaF）的水15天、30天和2个月，建立氟中毒大鼠模型。制备Ⅱ型胶原α1（II）cDNA探针，采用cDNA-mRNA原位杂交法检测过量摄入氟（221mg/L NaF）大鼠骨组织中Ⅱ型胶原mRNA表达的变化。采用天狼星偏振光法观察骨组织中胶原的类型和胶原纤维的形态。
氟暴露后大鼠股骨等骨组织中出现软骨细胞。cDNA-mRNA原位杂交显示，在软骨细胞陷窝和软骨化骨组织的细胞质中可观察到Ⅱ型胶原基因的表达。氟暴露15天后，肋软骨细胞胶原中的mRNA达到峰值水平，暴露1个月和2个月后逐渐下降。采用天狼星偏振光法可发现骨组织中Ⅱ型胶原多色性、胶原纤维断裂、排列紊乱及Ⅱ型胶原含量减少。
过量摄入氟可导致骨组织胶原（交联）类型和结构的改变，引起软骨化骨组织中Ⅱ型胶原基因的表达，并增强其在肋软骨组织中的表达。
