Liu Ying, Chen Dong, Chen Ai-jun, Nie Yu-xiu, Lu Ran, Ni Jia-zuan
Department of Histology and Embryology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Jul;37(4):263-5.
To probe the effects of long-term oral administration of lanthanum nitrate [La(NO(3))(3)] on morphological change in the liver, aftereffect of deposited La in the liver and their mechanism in rats.
Young Wistar rats were divided into two groups, one fed with 0.1, 0.2, 2.0, 10.0 and 20.0 mg/kg of La(NO(3))(3) for six months and the other for the control. Changes in ratio of liver to body weight were observed after exposure to La(NO(3))(3) at varied doses for six months and one month after six-month exposure, as well as morphology of the liver in the rats with routine histochemistry and transmission electron microscopy (TEM) technique. Content of La in the liver was measured with inductively coupled plasma-mass spectrometry (ICP-MS).
Ratio of liver to body weight was significantly higher in the male rats exposed to 20.0 mg/kg of lanthanum for six months than that in the control group. Ratio of liver to body weight restored to normal in the rats exposed to 20.0 mg/kg of La one month after six-month exposure. Infiltration of inflammatory cells in the portal region of the liver, small amount of fat drops in hepatocytic cytoplasm, increased density of mitochondria stroma, lysosome containing highly-electronic-density bodies and dense granules, normal nucleus and slightly deformed nucleus of hepatocytes could be found in the rats exposed to 20.0 mg/kg. Areas of the liver deposited with glycogen after six-month exposure to 20.0 mg/kg of La accounted for (26.1 +/- 1.5)% and (4.1 +/- 1.4)%, respectively for male and female rats, significantly lower than those in the control group [(31.3 +/- 1.4)% and (39.4 +/- 0.9)%, respectively], with a statistical significance and very statistical significance, respectively. There was a little infiltration of inflammatory cells in the portal region of the liver one month after six-month exposure to 20.0 mg/kg of La, and amount of the dense bodies was lower in the rats exposed to La for six months. Liver contents of La in the rats of all experimental groups were lower one month after six-month exposure than those in the rats exposed for six months.
Exposure to a dose of 20.0 mg/kg La(NO(3))(3) for a long term could damage the liver structure to certain extent, but lanthanum deposited in the liver could be eliminated from the body gradually.
探讨长期口服硝酸镧[La(NO₃)₃]对大鼠肝脏形态变化、肝脏中镧沉积的后效应及其机制。
将幼年Wistar大鼠分为两组，一组分别给予0.1、0.2、2.0、10.0和20.0mg/kg的La(NO₃)₃，连续6个月，另一组作为对照组。观察不同剂量La(NO₃)₃暴露6个月后及6个月暴露结束后1个月大鼠肝脏与体重比值的变化，并用常规组织化学和透射电镜（TEM）技术观察大鼠肝脏形态。用电感耦合等离子体质谱（ICP-MS）测定肝脏中镧的含量。
雄性大鼠暴露于20.0mg/kg镧6个月后肝脏与体重比值显著高于对照组。暴露于20.0mg/kg La 6个月后1个月，大鼠肝脏与体重比值恢复正常。在暴露于20.0mg/kg的大鼠中，可发现肝门区炎性细胞浸润、肝细胞质内少量脂滴、线粒体基质密度增加、含有高电子密度体和致密颗粒的溶酶体、正常细胞核和轻度变形的肝细胞细胞核。暴露于20.0mg/kg La 6个月后，肝脏糖原沉积面积雄性大鼠和雌性大鼠分别占(26.1±1.5)%和(4.1±1.4)%，显著低于对照组[(31.3±1.4)%和(39.4±0.9)%]，分别具有统计学意义和非常显著的统计学意义。暴露于20.0mg/kg La 6个月后1个月，肝门区有少量炎性细胞浸润，暴露于镧6个月的大鼠中致密体数量较少。所有实验组大鼠6个月暴露结束后1个月肝脏中镧的含量均低于暴露6个月的大鼠。
长期暴露于20.0mg/kg La(NO₃)₃可对肝脏结构造成一定程度的损害，但肝脏中沉积的镧可逐渐从体内清除。
