Ye Lie-fu, Zhang Yuan-fang, Fang Zu-jun, Ding Qiang, Yao Meng-shu
Department of Urology, Fujian Provincial Hospital, Fuzhou 350001, China.
Zhonghua Yi Xue Za Zhi. 2003 Aug 10;83(15):1296-9.
To study the expression levels and cell-specific expression patterns of steroid 5alpha-reductase type 1 and type 2 isoenzymes in human benign prostatic hyperplasia (BPH) tissues.
Immunohistochemistry and RT-PCR were used to investigate qualitatively and semi-quantitatively the expression of 5alpha-reductases type 1 and type 2 in 15 specimens of normal prostate tissues from dead kidney donor or patients undergoing cystectomy and 25 BPH tissues obtained from suprapubie enucleation.
Both steroid 5alpha-reductase type 1 mRNA and protein and 5alpha-reductase type 2 mRNA and protein were present in the normal and BPH tissues, especially in the glandular epithelial cells and mainly in the cytoplasm. In the BPH group the expression of type 2 reductase in the periurethral tissues was significant higher than that in the subcapsular tissues (1.50 +/- 0.42 vs. 1.07 +/- 0.35, P < 0.01), however, there was no significant difference in the expression of type 1 reductase between these 2 zones (P > 0.05). The expression level of 5alpha-reductase type 2 mRNA in BPH tissues was significantly higher than that in the normal specimens (e.g. in periurethral zone 1.50 +/- 0.42 vs. 0.98 +/- 0.32, P < 0.01), but there was no statistically significant difference in the expression of 5alpha-reductase type 1 mRNA between these 2 groups (e.g. in periurethral zone 0.62 +/- 0.31 vs 0.51 +/- 0.24, P > 0.05). In BPH tissues, the expression of type 2 mRNA was significantly higher in the larger prostate tissues (>or= 40 g) than in the smaller ones (e.g. in periurethral zone 1.58 +/- 0.47 vs. 1.14 +/- 0.46, P < 0.05), however, there was no significant difference in the expression of 5alpha-reductase type 1 mRNA between the BPH tissues with different volumes.
Type 2 5alpha-reductase plays an important role in the pathogenesis and maintenance of BPH. The role of type 1 expression in the pathogenesis of BPH needs further research.
研究1型和2型类固醇5α-还原酶同工酶在人良性前列腺增生（BPH）组织中的表达水平及细胞特异性表达模式。
采用免疫组织化学和逆转录-聚合酶链反应（RT-PCR）定性和半定量研究15例来自肾移植供体死亡者或接受膀胱切除术患者的正常前列腺组织以及25例经耻骨上前列腺摘除术获得的BPH组织中1型和2型5α-还原酶的表达。
正常组织和BPH组织中均存在1型类固醇5α-还原酶的mRNA和蛋白以及2型5α-还原酶的mRNA和蛋白，尤其在腺上皮细胞中，且主要位于细胞质中。在BPH组中，尿道周围组织中2型还原酶的表达显著高于包膜下组织（1.50±0.42 vs. 1.07±0.35，P<0.01），然而，这两个区域中1型还原酶的表达无显著差异（P>0.05）。BPH组织中2型5α-还原酶mRNA的表达水平显著高于正常标本（如在尿道周围区域1.50±0.42 vs. 0.98±0.32，P<0.01），但这两组之间1型5α-还原酶mRNA的表达无统计学显著差异（如在尿道周围区域0.62±0.31 vs 0.51±0.24，P>0.05）。在BPH组织中，较大前列腺组织（≥40 g）中2型mRNA的表达显著高于较小组织（如在尿道周围区域1.58±0.47 vs. 1.14±0.46，P<0.05），然而，不同体积的BPH组织中1型5α-还原酶mRNA的表达无显著差异。
2型5α-还原酶在BPH的发病机制及维持过程中起重要作用。1型5α-还原酶表达在BPH发病机制中的作用有待进一步研究。
