[一步法逆转录聚合酶链反应检测横纹肌肉瘤中PAX3/PAX7-FKHR融合转录本]
[Detection of PAX3/PAX7-FKHR fusion transcript in rhabdomyosarcoma by one-step RT-PCR].
作者信息
Chang Bin, Yin Bao-min, Li Feng, Li Xin-xia, Lu Tian-cai, Hu Wen-hao, Jiang Jin-fang, Shi Da-ren
机构信息
Department of Pathology, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2003 Aug 10;83(15):1336-8.
OBJECTIVE
To detect the PAX3-FKHR and PAX7-FKHR fusion transcripts in formalin-fixed, paraffin-embedded rhabdomyosarcoma tissues by one-step RT-PCR and discuss its diagnostic potential.
METHODS
One-step RT-PCR were used to detect the expression of the PAX3-FKHR and PAX7-FKHR fusion transcripts in 15 cases of rhabdomyosarcoma (6 cases of ARMS, 9 cases of ERMS and 1 case of PRMS) and 15 cases of non-rhabdomyosarcomous small round cell tumor.
RESULTS
PAX3-FKHR and PAX7-FKHR fusion transcripts were positive in 3/6 and 1/6 of ARMS patients, respectively, and were all negative in ERMS, PRMS and Control tumors including 4 cases of synovial sarcoma,4 cases of Ewing's sarcoma,4 cases of lymphoma and 3 cases of neuroblastoma.
CONCLUSION
Expression of PAX3-FKHR and PAX7-FKHR fusion transcripts detected by one-step RT-PCR is useful in diagnosis and classification of rhabdomyosarcoma.
目的
通过一步法逆转录聚合酶链反应（RT-PCR）检测福尔马林固定、石蜡包埋的横纹肌肉瘤组织中PAX3-FKHR和PAX7-FKHR融合转录本，并探讨其诊断潜力。
方法
采用一步法RT-PCR检测15例横纹肌肉瘤（6例腺泡状横纹肌肉瘤、9例胚胎性横纹肌肉瘤和1例多形性横纹肌肉瘤）及15例非横纹肌肉瘤性小圆细胞肿瘤中PAX3-FKHR和PAX7-FKHR融合转录本的表达。
结果
PAX3-FKHR和PAX7-FKHR融合转录本在腺泡状横纹肌肉瘤患者中分别为3/6和1/6阳性，在胚胎性横纹肌肉瘤、多形性横纹肌肉瘤及对照肿瘤（包括4例滑膜肉瘤、4例尤因肉瘤、4例淋巴瘤和3例神经母细胞瘤）中均为阴性。
结论
通过一步法RT-PCR检测PAX3-FKHR和PAX7-FKHR融合转录本对横纹肌肉瘤的诊断和分类有帮助。
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