Park Jong Hyuk, Kim Sun Jong, Oh Eun Jeong, Moon Shin Yong, Roh Sung Il, Kim Chul Geun, Yoon Hyun Soo
Division of Stem Cell Biology, Medical Research Center, MizMedi Hospital, Kangseo-ku, Seoul 157-280, Korea.
Biol Reprod. 2003 Dec;69(6):2007-14. doi: 10.1095/biolreprod.103.017467. Epub 2003 Aug 20.
Human embryonic stem (hES) cells have been traditionally cultured on primary mouse embryonic fibroblasts (PMEFs). However, though STO cells have some advantages over PMEFs and human embryonic fibroblasts (hEFs) as feeder cells, they have never been used as feeder cells to establish hES cell lines. In this study, three hES cell lines (Miz-hES1, Miz-hES2, and Miz-hES3) were established from inner cell masses (ICM), using STO as feeder cells. The three hES cell lines had normal karyotypes and expressed high levels of alkaline phosphatase (AP), cell surface markers (SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), and transcription factor Oct-4. After culture on STO cells for 2 yr, hES cells maintained the potential to form derivatives of all three embryonic germ layers. Our results show that STO feeder cells have the potential to support the establishment and maintenance of hES cell lines. In addition, our results suggest that laminin may play an important role in maintaining the undifferentiated proliferation of hES cells.
人胚胎干细胞（hES细胞）传统上是在原代小鼠胚胎成纤维细胞（PMEF）上培养的。然而，尽管STO细胞作为饲养层细胞比PMEF和人胚胎成纤维细胞（hEF）有一些优势，但它们从未被用作饲养层细胞来建立hES细胞系。在本研究中，使用STO作为饲养层细胞，从内细胞团（ICM）建立了三个hES细胞系（Miz-hES1、Miz-hES2和Miz-hES3）。这三个hES细胞系具有正常的核型，表达高水平的碱性磷酸酶（AP）、细胞表面标志物（SSEA-3、SSEA-4、TRA-1-60和TRA-1-81）以及转录因子Oct-4。在STO细胞上培养2年后，hES细胞仍保持形成所有三个胚胎胚层衍生物的潜能。我们的结果表明，STO饲养层细胞有支持hES细胞系建立和维持的潜能。此外，我们的结果表明层粘连蛋白可能在维持hES细胞的未分化增殖中起重要作用。
