扩展遗传信息系统的酶促修复
Enzymatic repair of an expanded genetic information system.
作者信息
Moser Michael J, Prudent James R
机构信息
Eragen Biosciences, Inc., 918 Deming Way, Madison, WI 53717-1944, USA.
出版信息
Nucleic Acids Res. 2003 Sep 1;31(17):5048-53. doi: 10.1093/nar/gkg709.
Abstract
The excision repair machinery of a thermophilic bacterium has been shown to recognize and repair an expanded genetic base pair. Native Thermus aquaticus DNA polymerase will remove a mispaired natural base and replace it with a non-natural base to form an expanded base pair. In addition, DNA ligase will recognize a nick formed by polymerase between two non-natural base pairs and covalently attach the two strands, thus demonstrating complete repair of a bifurcated base-paired model duplex. These results add evidence to the idea that the cellular replication and repair machinery of an organism containing an expanded genetic alphabet could recognize and properly repair a site containing consecutive unnatural bases.
摘要
嗜热细菌的切除修复机制已被证明能够识别并修复扩展的基因碱基对。天然嗜热水生栖热菌DNA聚合酶会去除错配的天然碱基，并用非天然碱基取代它，以形成扩展碱基对。此外，DNA连接酶会识别聚合酶在两个非天然碱基对之间形成的切口，并将两条链共价连接起来，从而证明对分叉碱基配对模型双链体的完全修复。这些结果为以下观点提供了更多证据：含有扩展基因字母表的生物体的细胞复制和修复机制能够识别并正确修复包含连续非天然碱基的位点。
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