Olson M E, Gard S, Brown M, Hampton R, Morck D W
Department of Biological Sciences and Animal Care Services, University of Calgary, Alberta, Canada.
J Am Vet Med Assoc. 1992 Dec 1;201(11):1766-70.
An investigation of an epidemic of infectious disease in a frog (Rana pipiens) colony was conducted. Six of 40 frogs in a continuous (once through) water flow housing system had weight loss, swollen abdomen, corneal edema, uveitis, subcutaneous edema, petechial hemorrhage, incoordination, and respiratory distress. The frogs had lesions consistent with bacterial septicemia. A gram-negative, nonfermenting bacillus, Flavobacterium indologenes (Flavobacterium sp biovar IIb), was isolated in pure culture from tissues and blood. The clinical isolate was used to inoculate healthy frogs sc. An isolate identical to the one isolated from the sick frogs was recovered from tissues and blood of the inoculated frogs. Inoculation of the housing water in a nonflow-through system did not result in disease, despite proliferation of the Flavobacterium spp in the water; therefore, it is likely that establishment of infection requires the presence of the organism in sufficient numbers and a portal of entry into the body.
对一个青蛙（豹蛙）群落中的传染病疫情进行了调查。在一个连续（一次性通过）水流的饲养系统中的40只青蛙里，有6只出现体重减轻、腹部肿胀、角膜水肿、葡萄膜炎、皮下水肿、瘀点出血、共济失调和呼吸窘迫。这些青蛙的病变与细菌性败血症一致。从组织和血液中纯培养分离出一种革兰氏阴性、非发酵杆菌，吲哚黄杆菌（黄杆菌属生物变种IIb）。临床分离株用于皮下接种健康青蛙。从接种青蛙的组织和血液中回收了与从患病青蛙中分离出的菌株相同的分离株。尽管黄杆菌属在非流通系统的饲养水中大量繁殖，但在该系统中接种饲养水并未导致疾病；因此，感染的发生可能需要足够数量的病原体存在并进入机体的途径。
