Li Wenli, Jin Liji, An Lijia
Bioengineering Department, Dalian University of Technology, Dalian 116012, Liaoning Province, PR China.
DNA Cell Biol. 2003 Jul;22(7):441-6. doi: 10.1089/104454903322247316.
The transcription initiation site of Anrheraea pernyi was identified, and a gene targeting vector was constructed. The green fluorescent protein (GFP) gene was inserted into this vector under the control of a fibroin promoter. This recombinant vector was used to target the GFP gene to the fibroin region of the silkworm genome. The DNA was injected into the testes during the pupae, and was also transferred into the egg via the sperm during fertilization. The analysis showed that the GFP gene was integrated into the fibroin gene on the genome by homologous recombination, and was expressed in the silk gland.
