恶性疟原虫：来自哥伦比亚两个不同流行程度地区的分离株的多样性研究。

Plasmodium falciparum: diversity studies of isolates from two Colombian regions with different endemicity.

作者信息

Montoya L, Maestre A, Carmona J, Lopes D, Do Rosario V, Blair S

机构信息

Grupo Malaria, Laboratorio de Hemoparásitos, Faculty of Medicine, University of Antioquia, Carrera 51D # 62-29, AA:1226, Medellín, Colombia.

出版信息

Exp Parasitol. 2003 May-Jun;104(1-2):14-9. doi: 10.1016/s0014-4894(03)00112-7.

DOI:10.1016/s0014-4894(03)00112-7

PMID:12932754

Abstract

The population structure of Plasmodium falciparum has been widely studied in diverse epidemiological contexts, but emphasis has been made in regions with high and stable transmission. In order to establish the genetic structure of P. falciparum in areas of Colombia with different degree of endemicity, we studied 100 samples from malaria patients of two different municipalities. The frequency of multiclonal infection in these areas and the correlation with the endemicity were carried out by comparison of the amplified products from polymorphic segments of MSP-1, MSP-2, and GLURP genes. We found low size polymorphism of the studied genes: 1 MSP-1 allele, 3 MSP-2 alleles, and 4 GLURP alleles. We conclude that the P. falciparum population in the regions studied is genetically homogeneous.

摘要

恶性疟原虫的种群结构已在不同的流行病学背景下得到广泛研究，但重点一直放在传播率高且稳定的地区。为了确定哥伦比亚不同流行程度地区恶性疟原虫的遗传结构，我们研究了来自两个不同城市的疟疾患者的100份样本。通过比较MSP-1、MSP-2和GLURP基因多态性片段的扩增产物，对这些地区的多克隆感染频率及其与流行程度的相关性进行了研究。我们发现所研究基因的大小多态性较低：1个MSP-1等位基因、3个MSP-2等位基因和4个GLURP等位基因。我们得出结论，所研究地区的恶性疟原虫种群在遗传上是同质的。