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印度东北部特里普拉邦疟原虫寄生虫的耐药性和遗传多样性特征。

Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India.

机构信息

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

Model Rural Health Research Unit (DHR), Assam, India.

出版信息

Sci Rep. 2019 Sep 23;9(1):13704. doi: 10.1038/s41598-019-50152-w.

DOI:10.1038/s41598-019-50152-w
PMID:31548652
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6757058/
Abstract

Monitoring of anti-malarial drug resistance is vital in Northeast India as this region shares its international border with Southeast Asia. Genetic diversity of Plasmodium parasites regulates transmission dynamics, disease severity and vaccine efficacy. P. falciparum chloroquine resistance transporter (Pfcrt), multidrug resistance-1 (Pfmdr-1) and kelch 13 propeller (PfK-13) genes which govern antimalarial drug resistance and three genetic diversity markers, merozoite surface protein 1 and 2 (Pfmsp-1, Pfmsp-2) and glutamate rich protein (Pfglurp) were evaluated from Tripura, Northeast India using molecular tools. In the Pfcrt gene, 87% isolates showed triple mutations at codons M74I, N75E and K76T. 12.5% isolates in Pfmdr-1 gene showed mutation at N86Y. No polymorphism in PfK-13 propeller was found. Polyclonal infections were observed in 53.85% isolates and more commonly in adults (p = 0.0494). In the Pfmsp-1 locus, the K1 allelic family was predominant (71.2%) followed by the 3D7/IC family (69.2%) in the Pfmsp-2 locus. RII region of Pfglurp exhibited nine alleles with expected heterozygosity of 0.85. The multiplicity of infection for Pfmsp-1, Pfmsp-2 and Pfglurp were 1.56, 1.31 and 1.06 respectively. Overall, the study demonstrated a high level of chloroquine resistance and extensive parasite diversity in the region, necessitating regular surveillance in this population group.

摘要

在印度东北部，由于该地区与东南亚接壤，因此监测抗疟药物耐药性至关重要。疟原虫寄生虫的遗传多样性调节着传播动力学、疾病严重程度和疫苗效果。氯喹耐药转运蛋白（Pfcrt）、多药耐药-1（Pfmdr-1）和 Kelch13 螺旋桨（PfK-13）基因控制着抗疟药物耐药性，以及三种遗传多样性标记，即裂殖子表面蛋白 1 和 2（Pfmsp-1、Pfmsp-2）和谷氨酸丰富蛋白（Pfglurp），这些基因在印度东北部的特里普拉邦使用分子工具进行了评估。在 Pfcrt 基因中，87%的分离株在密码子 M74I、N75E 和 K76T 处显示三重突变。Pfmdr-1 基因中有 12.5%的分离株显示 N86Y 突变。PfK-13 螺旋桨没有发现多态性。在 53.85%的分离株中观察到多克隆感染，在成年人中更为常见（p=0.0494）。在 Pfmsp-1 基因座中，K1 等位基因家族占主导地位（71.2%），其次是 Pfmsp-2 基因座中的 3D7/IC 家族（69.2%）。Pfglurp 的 RII 区有九个等位基因，预期杂合度为 0.85。Pfmsp-1、Pfmsp-2 和 Pfglurp 的感染多重性分别为 1.56、1.31 和 1.06。总的来说，该研究表明该地区存在高水平的氯喹耐药性和广泛的寄生虫多样性，因此需要对该人群进行定期监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/a5836d94540c/41598_2019_50152_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/dd4b27bdac5a/41598_2019_50152_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/1ee0606b6402/41598_2019_50152_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/2d6afaaff3f5/41598_2019_50152_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/6c561054d00e/41598_2019_50152_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/f35fb694139d/41598_2019_50152_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/a5836d94540c/41598_2019_50152_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/dd4b27bdac5a/41598_2019_50152_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/1ee0606b6402/41598_2019_50152_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/2d6afaaff3f5/41598_2019_50152_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/6c561054d00e/41598_2019_50152_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/f35fb694139d/41598_2019_50152_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b98/6757058/a5836d94540c/41598_2019_50152_Fig6_HTML.jpg

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