Buzzard Jeremy J, Wreford Nigel G, Morrison John R
Monash Institute of Reproduction and Development and Department of Anatomy and Cell Biology, Monash University, Clayton, 3168, Melbourne, Australia.
Endocrinology. 2003 Sep;144(9):3722-31. doi: 10.1210/en.2003-0379.
This study uses a high purity cell culture system to extend previous observations of factors controlling the end of the Sertoli cell proliferative phase. Thyroid hormone, retinoic acid, and testosterone were assessed for their ability to halt the proliferative phase and regulate the expression of markers associated with maturation of the Sertoli cell. We show that these hormones share similar suppressive effects on the rate of Sertoli cell division without any apparent additive effects. We demonstrate that these hormones induce the progressive accumulation of cell cycle inhibitors p27Kip1 and p21Cip1 in Sertoli cells, a likely regulatory mechanism controlling the suppression of proliferation. We used real-time RT-PCR to examine the effects of these factors on the expression of mRNA encoding the Id proteins, demonstrating an increase in Id2 and Id3 expression in Sertoli cells treated with thyroid hormone, retinoic acid, or testosterone. Finally, we examined the expression of a number of genes that have been implicated in the Sertoli cell differentiation process. Our results suggest that these hormones can induce aspects of Sertoli cell differentiation in vitro, providing a valuable in vitro model for studying Sertoli cell function.
本研究采用高纯度细胞培养系统，以拓展先前对控制支持细胞增殖期结束的因素的观察。评估了甲状腺激素、视黄酸和睾酮阻止增殖期以及调节与支持细胞成熟相关标志物表达的能力。我们发现这些激素对支持细胞分裂速率具有相似的抑制作用，且无明显的叠加效应。我们证明这些激素诱导支持细胞中细胞周期抑制剂p27Kip1和p21Cip1的逐步积累，这可能是控制增殖抑制的一种调节机制。我们使用实时逆转录聚合酶链反应来检测这些因素对编码Id蛋白的mRNA表达的影响，结果表明在用甲状腺激素、视黄酸或睾酮处理的支持细胞中，Id2和Id3表达增加。最后，我们检测了一些与支持细胞分化过程相关的基因的表达。我们的结果表明，这些激素可在体外诱导支持细胞分化的某些方面，为研究支持细胞功能提供了一个有价值的体外模型。
