Hu Dong-Liang, Omoe Katsuhiko, Sasaki Sanae, Sashinami Hiroshi, Sakuraba Hirotake, Yokomizo Yuichi, Shinagawa Kunihiro, Nakane Akio
Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki, Japan.
J Infect Dis. 2003 Sep 1;188(5):743-52. doi: 10.1086/377308. Epub 2003 Aug 14.
To investigate whether vaccination with nontoxic mutant toxic shock syndrome toxin 1 (mTSST-1) can protect against Staphylococcus aureus infection, mice were vaccinated with mTSST-1 and challenged with viable S. aureus. Survival in the mTSST-1-vaccinated group was higher, and bacterial counts in organs were significantly lower than those of control mice. Passive transfer of mTSST-1-specific antibodies also provided protection against S. aureus-induced septic death. Interferon (IFN)-gamma production in the serum samples and spleens from vaccinated mice was significantly decreased compared with that in controls, whereas interleukin-10 titers were significantly higher in vaccinated mice. IFN-gamma and tumor necrosis factor-alpha production in vitro were significantly inhibited by serum samples from mTSST-1-immunized mice but not from control mice. These results suggest that vaccination with mTSST-1 devoid of superantigenic properties provides protection against S. aureus infection and that the protection might be mediated by TSST-1-neutralizing antibodies as well as by the down-regulation of IFN-gamma production.
为了研究接种无毒突变体中毒性休克综合征毒素1（mTSST-1）是否能预防金黄色葡萄球菌感染，给小鼠接种mTSST-1并使其感染活的金黄色葡萄球菌。接种mTSST-1的小鼠存活率更高，且器官中的细菌数量显著低于对照小鼠。mTSST-1特异性抗体的被动转移也能预防金黄色葡萄球菌诱导的败血症死亡。与对照相比，接种疫苗小鼠血清样本和脾脏中干扰素（IFN）-γ的产生显著减少，而接种疫苗小鼠白细胞介素-10滴度显著更高。mTSST-1免疫小鼠的血清样本可显著抑制体外IFN-γ和肿瘤坏死因子-α的产生，但对照小鼠的血清样本则无此作用。这些结果表明，接种无超抗原特性的mTSST-1可预防金黄色葡萄球菌感染，且这种保护作用可能由TSST-1中和抗体以及IFN-γ产生的下调介导。
