Nishida S, Ohkuma M
Department of Dermatology, Kinki University, School of Medicine, Osaka, Japan.
Lymphology. 1992 Dec;25(4):184-90.
This investigation was performed in order to establish a new histochemical method for the identification of lymphatic capillaries. The microvasculature in specimens from human foreskin was examined for adenylate cyclase and alkaline phosphatase activity by light and electron microscopy. Lymphatic capillaries showed positive adenylate cyclase reactivity and negative alkaline phosphatase reactivity whereas the blood capillaries showed a positive reaction for alkaline phosphatase and a negative one for adenylate cyclase. The presence of adenylate cyclase activity in the endothelium of the lymphatic capillary may relate to microvascular function including the transcapillary exchange of water and solutes.
进行这项研究是为了建立一种用于识别淋巴管的新组织化学方法。通过光学显微镜和电子显微镜检查人包皮标本中的微血管，以检测腺苷酸环化酶和碱性磷酸酶活性。淋巴管显示出腺苷酸环化酶反应阳性和碱性磷酸酶反应阴性，而毛细血管则显示碱性磷酸酶反应阳性和腺苷酸环化酶反应阴性。淋巴管内皮中腺苷酸环化酶活性的存在可能与微血管功能有关，包括水和溶质的跨毛细血管交换。
