Li Guo-liang, Xiao Bo, Xie Guang-jie
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
Hunan Yi Ke Da Xue Xue Bao. 2003 Feb 28;28(1):29-32.
To characterize the acute and chronic behavioral, electrographic and histological changes of sustained seizures induced by pilocarpine in rats.
The rats in the study were divided into the experimental group and control group. After status epilepticus (SE) was induced in the experimental rats, the surviving animals were continuously monitored for 6 h-60 days. At different times after the pilocarpine injection, the animals were processed for neo-Timm and Nissl staining to visualize granule cell mossy fiber sprouting and hippocampal cell damage.
Of the animals injected with pilocarpine, 87% developed SE, and most of the pilocarpine-induced SE rats (20%-100%) showed recurrent seizures during the chronic period. A widespread cell loss was noted in the hippocampal formation of the rats with pilocarpine-induced SE. The pattern of neo-Timm staining in the inner molecular layer was clearly altered in animals that showed pilocarpine-induced SE. The Neo-Timm staining score of the experimental group was significantly higher than that of the control group. The degree of inner molecular layer mossy fiber terminal staining increased with time after the epileptogenic lesion.
The temporal lobe epilepsy model induced by pilocarpine in rats can replicate several of the features of human temporal epilepsy (hippocampal cell loss, inner molecular layer mossy fiber sprouting, and spontaneous recurrent seizures), and it may be a useful model for studying this human temporal lobe epilepsy. The results also suggest that structural brain damage insulted by pilocarpine-induced SE may underlie or be associated with recurrent spontaneous seizures in rats.
描述匹鲁卡品诱导大鼠持续性癫痫发作后的急性和慢性行为、脑电图及组织学变化。
将研究中的大鼠分为实验组和对照组。在实验组大鼠诱发癫痫持续状态（SE）后，对存活的动物连续监测6小时至60天。在注射匹鲁卡品后的不同时间，对动物进行新Timm染色和尼氏染色，以观察颗粒细胞苔藓纤维发芽和海马细胞损伤情况。
注射匹鲁卡品的动物中，87%出现SE，且大多数匹鲁卡品诱导的SE大鼠（20%-100%）在慢性期出现反复癫痫发作。在匹鲁卡品诱导SE的大鼠海马结构中观察到广泛的细胞丢失。在出现匹鲁卡品诱导SE的动物中，内分子层新Timm染色模式明显改变。实验组的新Timm染色评分显著高于对照组。癫痫病灶形成后，内分子层苔藓纤维终末染色程度随时间增加。
匹鲁卡品诱导的大鼠颞叶癫痫模型可复制人类颞叶癫痫的一些特征（海马细胞丢失、内分子层苔藓纤维发芽和自发性反复癫痫发作），可能是研究人类颞叶癫痫的有用模型。结果还表明，匹鲁卡品诱导的SE所致的脑结构损伤可能是大鼠反复自发性癫痫发作的基础或与之相关。
