Liu Chang-yun, Ji Hong-guang, Zhou Jian-guang, Qian Ping-ping, Shen Hui, Ruan Fang-ming
Naval Hospital No. 411 of People's Liberation Army, Shanghai, China.
Space Med Med Eng (Beijing). 2003 Jun;16(3):175-8.
To study the effects of rapid eye movement sleep (REMS) deprivation on the expression of mRNA coding for neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) of hypothalamus in rats.
Flower pot technique was adopted to deprive the REMS of Sprague-Dawley rats for 24 h, 48 h and 72 h respectively. The expression of mRNA coding for nNOS or iNOS of hypothalamus in rats was assayed by reverse-transcription polymerase chain reaction (RT-PCR).
The amount of nNOS mRNA was significantly higher in 24 h REMS deprivation group (P<0.01), then the amount was lowered in 48 h deprivation group and became significantly lower in 72 h deprivation group than that in the control group (P<0.05). There was low expression of iNOS mRNA of hypothalamus in rats, and there was no difference in the expression of iNOS mRNA among 24 h, 48 h REMS deprivation and the control groups. But the expression was significantly increased in 72 h REMS deprivation group (P<0.01).
Deprivation of REMS increased the expression of nNOS and iNOS mRNA of hypothalamus. Excessive nitric oxide (NO) might be a major factor resulting in not only the sleep rebound phenomenon but also the injury of human function caused by sleep loss directly or indirectly by other sleep factors.
研究快速眼动睡眠（REMS）剥夺对大鼠下丘脑神经元型一氧化氮合酶（nNOS）和诱导型一氧化氮合酶（iNOS）编码mRNA表达的影响。
采用花盆法分别剥夺Sprague-Dawley大鼠24小时、48小时和72小时的快速眼动睡眠。通过逆转录聚合酶链反应（RT-PCR）检测大鼠下丘脑nNOS或iNOS编码mRNA的表达。
24小时快速眼动睡眠剥夺组nNOS mRNA量显著升高（P<0.01），48小时剥夺组该量降低，72小时剥夺组比对照组显著降低（P<0.05）。大鼠下丘脑iNOS mRNA表达较低，24小时、48小时快速眼动睡眠剥夺组与对照组之间iNOS mRNA表达无差异。但72小时快速眼动睡眠剥夺组表达显著增加（P<0.01）。
快速眼动睡眠剥夺增加了下丘脑nNOS和iNOS mRNA的表达。过量的一氧化氮（NO）可能是不仅导致睡眠反弹现象，而且直接或间接由其他睡眠因素导致睡眠剥夺引起人体功能损伤的主要因素。
