Iqbal Mohammad, Noor Rana, Mizuno Ryuichiro, Okada Shigeru
Department of Pathological Research, Faculty of Medicine, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Redox Rep. 2003;8(3):163-7. doi: 10.1179/135100003225001557.
Several studies have shown the role of thiol-rich proteins especially metallothionein (MT) in the therapeutic interventions against oxidative damage. Previously, we have provided strong evidence for the involvement of ROS in iron nitrilotriacetate (Fe-NTA)-induced renal toxicity, which may have relevance to its carcinogenicity. The purpose of this study was to evaluate the role of zinc metallothionein (Zn-MT) on the protection against Fe-NTA-induced renal oxidative damage. The results demonstrate that Zn-MT pretreatment provided protection against Fe-NTA-induced mortality in mice (40% protection). Similarly, Zn-MT pretreatment also provided protection against Fe-NTA-induced lipid peroxidation (26% inhibition, P < 0.001). It is proposed that Zn-MT protects kidney tissue against the noxious effect of Fe-NTA primarily by interference with lipid peroxides. It is concluded that Zn-MT may serve as an excellent physiological antioxidant against Fe-NTA-mediated renal oxidative damage.
多项研究表明，富含硫醇的蛋白质尤其是金属硫蛋白（MT）在针对氧化损伤的治疗干预中所起的作用。此前，我们已提供有力证据证明活性氧（ROS）参与了次氮基三乙酸铁（Fe-NTA）诱导的肾毒性，这可能与其致癌性有关。本研究的目的是评估锌金属硫蛋白（Zn-MT）在预防Fe-NTA诱导的肾氧化损伤中的作用。结果表明，Zn-MT预处理可预防Fe-NTA诱导的小鼠死亡（保护率为40%）。同样，Zn-MT预处理也可预防Fe-NTA诱导的脂质过氧化（抑制率为26%，P<0.001）。有人提出，Zn-MT主要通过干扰脂质过氧化物来保护肾组织免受Fe-NTA的有害影响。得出的结论是，Zn-MT可能作为一种出色的生理性抗氧化剂，对抗Fe-NTA介导的肾氧化损伤。
