使用AmpFLSTR Profiler试剂盒对陈旧精液斑进行九个短串联重复序列位点分析及一种新的vWA变异等位基因的描述
Nine short tandem repeat loci analysis in aged semen stains using the AmpFLSTR Profiler Kit and description of a new vWA variant allele.
作者信息
Kido Akira, Hara Masaaki, Yamamoto Yasuhisa, Kameyama Hiroshi, Susukida Rie, Saito Kazuyuki, Takada Aya, Oya Masakazu
机构信息
Department of Legal Medicine, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi-ken 409-3898, Japan.
出版信息
Leg Med (Tokyo). 2003 Jun;5(2):93-6. doi: 10.1016/s1344-6223(03)00009-9.
Nine short tandem repeat (STR) loci, D3S1358, D5S818, vWA, TH01, D13S317, TPOX, FGA, D7S820 and CSF1PO, were investigated in semen stains of various ages using the AmpFLSTR Profiler Kit. The nine STR loci were typed from semen stains stored for up to 25 years with the application of 1-10 ng DNA. This system provides a useful tool in medicolegal individualization of aged semen stains. During this investigation we found a new variant allele 18.1 at the vWA locus.
使用AmpFLSTR Profiler试剂盒，对不同年龄精液斑中的9个短串联重复序列（STR）基因座D3S1358、D5S818、vWA、TH01、D13S317、TPOX、FGA、D7S820和CSF1PO进行了研究。利用1-10 ng DNA，对保存长达25年的精液斑中的9个STR基因座进行了分型。该系统为老化精液斑的法医个体识别提供了一个有用的工具。在本研究中，我们在vWA基因座发现了一个新的变异等位基因18.1。
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