脱氧核糖核酸酶I（DNase I）多态性在从陈旧小尿渍中进行个体识别方面的实用性。
Usefulness of deoxyribonuclease I (DNase I) polymorphism for individualization from small aged urine stains.
作者信息
Yasuda Toshihiro, Takeshita Haruo, Ueki Misuzu, Nakajima Tamiko, Mogi Koichi, Kaneko Yasushi, Iida Reiko, Kishi Koichiro
机构信息
Department of Biology, Fukui Medical University, 910-1193, Fukui, Japan.
出版信息
Leg Med (Tokyo). 2003 Jun;5(2):105-7. doi: 10.1016/s1344-6223(03)00049-x.
We devised a procedure that combines a simple extraction method, isoelectric focusing and activity staining using the dried agarose film overlay method, for deoxyribonuclease I (DNase I) typing from aged urine stains. DNase I types were determined without difficulty from urine stains kept at room temperature for 3 months or more in all of the samples tested. The amounts of urine stains required for typing after 3 months of storage were estimated to be equivalent to 60-120 microl of liquid urine. Therefore, considering that useful PCR-based DNA typing has not yet been developed for urine stains, DNase I polymorphism could be considered the first biochemical marker found to be well suited for individualization from small aged urine stains.
我们设计了一种方法，该方法结合了简单的提取方法、等电聚焦以及使用干燥琼脂糖膜覆盖法的活性染色，用于对陈旧尿渍进行脱氧核糖核酸酶I（DNase I）分型。在所有测试样本中，从室温保存3个月或更长时间的尿渍中不难确定DNase I类型。储存3个月后进行分型所需的尿渍量估计相当于60 - 120微升液态尿液。因此，鉴于尚未开发出适用于尿渍的基于聚合酶链反应（PCR）的有效DNA分型方法，DNase I多态性可被视为首个被发现非常适合从小块陈旧尿渍中进行个体识别的生化标记。
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