Costello L C, Franklin R, Stacey R
Enzyme. 1976;21(6):495-506. doi: 10.1159/000458902.
Mitochondrial preparations isolated from rat ventral prostate were capable of oxidizing isocitrate by way of NADP isocitrate dehydrogenase (NADP-IDH) and NAD-IDH. NAD-IDH activity required ADP for activation. The pH responses for NAD-IDH and NADP-IDH were quite different. The results indicated that two different enzymes were involved in the NAD- and NADP-IDH activities. Indirect evidence indicated that NADPH-NAD transhydrogenase activity might also be involved in the mitochondrial pathway for isocitrate oxidation. NADP-IDH activity was significantly greater than NAD-IDH activity. The oxidation of isocitrate through IDH activity was coupled to the cytochrome system by NADPH- and NADH-cytochrome c reductase activities. Citrate, via isocitrate, oxidation proceeded at a much slower rate suggesting that aconitase activity could be limiting in the oxidation of citrate. In comparison to other tissues, the prostate oxidative enzyme activities are considerably lower. The results suggest that the accumulation of high prostate citrate levels is not due to a limitation imposed by a lack of IDH activity in prostate mitochondria.
从大鼠腹侧前列腺分离得到的线粒体制剂能够通过NADP异柠檬酸脱氢酶（NADP-IDH）和NAD-IDH氧化异柠檬酸。NAD-IDH活性需要ADP来激活。NAD-IDH和NADP-IDH的pH反应截然不同。结果表明，NAD-和NADP-IDH活性涉及两种不同的酶。间接证据表明，NADPH-NAD转氢酶活性可能也参与了异柠檬酸氧化的线粒体途径。NADP-IDH活性显著高于NAD-IDH活性。通过IDH活性进行的异柠檬酸氧化通过NADPH-和NADH-细胞色素c还原酶活性与细胞色素系统偶联。柠檬酸通过异柠檬酸氧化的速率要慢得多，这表明乌头酸酶活性可能是柠檬酸氧化的限制因素。与其他组织相比，前列腺的氧化酶活性相当低。结果表明，前列腺中高柠檬酸水平的积累并非由于前列腺线粒体中缺乏IDH活性所造成的限制。
