Chen Z F, Ma J T, Ni Z M, Mu Z W
Shanghai Institute of Physiology, Academia Sinica.
Sheng Li Xue Bao. 1992 Aug;44(4):370-8.
In view of the fact that in embryonic and neonatal central nervous system (CNS), the pathway of developing fiber tracts is capable of guiding the axonal growth, it would be interesting to know whether a similar effect exists on the axonal growth in adult CNS. Embryonic fimbria was grafted into the hippocampus of the adult rat. Two weeks later, the grafts were examined for cholinergic fibers with AChE histochemical method. It was found that a lot of cholinergic fibers appeared in the embryonic graft, but none of them in the adult fimbria graft as control. If the fimbria-fornix was transected at the time of grafting, no cholinergic fibers could subsequently be detected in both the embryonic graft and the host hippocampus. If a suspension of embryonic fimbria was used as a graft, only a few of long cholinergic fibers could be found in the grafted area. However, if tissue fragments of embryonic fimbria adhered to a strip of nitrocellulose filter were grafted as previously, numerous cholinergic fibers from the host hippocampus were found to be attracted around the strip and grow along the surface of the filter. The results seem to indicate that grafted embryonic fimbria or its tissue fragments are able to guide cholinergic fiber growth in adult hippocampus. It is possible that embryonic fimbria and other pathways of developing CNS fiber tracts provide a natural substrate for guiding axonal growth in adult CNS.
鉴于在胚胎和新生儿中枢神经系统（CNS）中，发育中的纤维束通路能够引导轴突生长，那么了解在成体CNS中对轴突生长是否存在类似作用将是很有意思的。将胚胎海马伞移植到成年大鼠的海马中。两周后，用乙酰胆碱酯酶（AChE）组织化学方法检查移植组织中的胆碱能纤维。结果发现，胚胎移植组织中有大量胆碱能纤维出现，但作为对照的成年海马伞移植组织中则没有。如果在移植时切断穹窿海马伞，随后在胚胎移植组织和宿主海马中均检测不到胆碱能纤维。如果用胚胎海马伞悬液作为移植组织，在移植区域只能发现少数长胆碱能纤维。然而，如果像以前那样将附着在一条硝酸纤维素滤膜上的胚胎海马伞组织碎片进行移植，则发现宿主海马中的大量胆碱能纤维被吸引到滤膜条周围并沿滤膜表面生长。这些结果似乎表明，移植的胚胎海马伞或其组织碎片能够引导成年海马中胆碱能纤维的生长。胚胎海马伞和中枢神经系统其他发育中的纤维束通路有可能为引导成体中枢神经系统中的轴突生长提供了天然基质。
