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[检测压缩粪便样本中曼氏血吸虫卵的效率]

[The efficiency of the examination of compressed fecal samples for Schistosoma mansoni eggs].

作者信息

Teles Horacio Manuel Santana, Ferreira Cláudio Santos, de Carvalho Maria Esther, Zacharias Fabiana, Magalhães Luiz Augusto

机构信息

Superintendência de Controle de Endemias, São Paulo, SP, Brasil.

出版信息

Rev Soc Bras Med Trop. 2003 Jul-Aug;36(4):503-7. doi: 10.1590/s0037-86822003000400012. Epub 2003 Aug 13.

Abstract

The control of schistosomiasis depends mostly upon the sensitivity of stool examinations. We used both the Kato-Katz technique and the compression of samples between the slide and a polypropylene cover glass, according to Ferreira. Out of 1,282 samples collected between 1998 and 2000 in the Palha District, Municipality of Bananal, São Paulo State, Brazil, 105 (8.2 %) were positive. The first and second methods revealed 90 (7%) and 88 (6.9 %) positive cases, respectively. According to the kappa statistic, both methods were in agreement. In Bananal, the proportion of positive cases and egg per gram (epg) values are low, which calls for the examination of larger than usual volumes of feces. The viability of Schistosoma mansoni eggs can also be checked when using the second method, which dispenses with glycerin solution, measuring plates and sieves. The latter cause a further loss of precision in epg determinations.

摘要

血吸虫病的控制主要取决于粪便检查的灵敏度。根据费雷拉的方法,我们同时使用了加藤厚涂片法以及在载玻片和聚丙烯盖玻片之间对样本进行挤压的方法。在巴西圣保罗州巴纳纳尔市帕尔哈区1998年至2000年期间采集的1282份样本中,有105份(8.2%)呈阳性。第一种和第二种方法分别检测出90例(7%)和88例(6.9%)阳性病例。根据卡帕统计量,两种方法结果一致。在巴纳纳尔,阳性病例比例和每克粪便虫卵数(epg)值都很低,这就需要检查比平常更多量的粪便。使用第二种方法时,还可以检查曼氏血吸虫卵的活力,该方法无需甘油溶液、量板和筛子。后三者会导致epg测定的精度进一步下降。

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