Coelho C, Vinatea C E B, Heinert A P, Simões C M O, Barardi C R M
Departamento de Farmácia, CCS, Universidade do Vale de Itajaí, Itajaí, SC, Brasil.
Mem Inst Oswaldo Cruz. 2003 Jun;98(4):465-8. doi: 10.1590/s0074-02762003000400006. Epub 2003 Aug 18.
Outbreaks of gastroenteritis have occurred among consumers of raw or undercooked shellfish harvested from faecally polluted waters. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) was applied for the simultaneous detection of hepatitis A virus (HAV), poliovirus (PV) and simian rotavirus (RV-SA11) and compared with specific primers for each genome sequence. Three amplified DNA products representing HAV (192 bp), PV (394 bp) and RV (278 bp) were identified when positive controls were used. However, when tested on experimentally contaminated raw oysters, this method was not able to detect the three viruses simultaneously. This is probably due to the low concentration of viral RNAs present in oyster extract which were partially lost during the extracts preparation.
食用从受粪便污染水域捕捞的生的或未煮熟的贝类的消费者中曾爆发肠胃炎。采用多重逆转录聚合酶链反应（RT-PCR）同时检测甲型肝炎病毒（HAV）、脊髓灰质炎病毒（PV）和猴轮状病毒（RV-SA11），并与针对每个基因组序列的特异性引物进行比较。使用阳性对照时，鉴定出了代表HAV（192 bp）、PV（394 bp）和RV（278 bp）的三种扩增DNA产物。然而，在对实验污染的生牡蛎进行检测时，该方法无法同时检测到这三种病毒。这可能是由于牡蛎提取物中存在的病毒RNA浓度较低，在提取物制备过程中部分丢失了。
