Westphalen R I, Scott H L, Dodd P R
Department of Biochemistry, University of Queensland, Brisbane, Australia.
J Neural Transm (Vienna). 2003 Sep;110(9):1013-27. doi: 10.1007/s00702-003-0012-z.
The apparent l-[3H]glutamate uptake rate (v') was measured in synaptic vesicles isolated from cerebral cortex synaptosomes prepared from autopsied Alzheimer and non-Alzheimer dementia cases, and age-matched controls. The initial synaptosome preparations exhibited similar densities of d-[3H]aspartate membrane binding sites (BMAX values) in the three groups. In control brain the temporal cortex d-[3H]aspartate BMAX was 132% of that in motor cortex, parallel with the l-[3H]glutamate v' values (temporal=139% of motor; NS). Unlike d-[3H]aspartate BMAX values, l-[3H]glutamate v' values were markedly and selectively lower in Alzheimer brain preparations than in controls, particularly in temporal cortex. The difference could not be attributed to differential effects of autopsy interval or age at death. Non-Alzheimer dementia cases resembled controls. The selective loss of vesicular glutamate transport is consistent with a dysfunction in the recycling of transmitter glutamate.
在从阿尔茨海默病和非阿尔茨海默病痴呆症尸检病例以及年龄匹配的对照制备的大脑皮质突触体中分离出的突触小泡中，测量了表观l-[3H]谷氨酸摄取率（v'）。最初的突触体制备物在三组中显示出相似密度的d-[3H]天冬氨酸膜结合位点（BMAX值）。在对照脑中，颞叶皮质d-[3H]天冬氨酸BMAX是运动皮质的132%，与l-[3H]谷氨酸v'值平行（颞叶=运动皮质的139%；无显著性差异）。与d-[3H]天冬氨酸BMAX值不同，阿尔茨海默病脑制备物中的l-[3H]谷氨酸v'值明显且选择性地低于对照，尤其是在颞叶皮质。这种差异不能归因于尸检间隔或死亡年龄的不同影响。非阿尔茨海默病痴呆症病例与对照相似。囊泡谷氨酸转运的选择性丧失与递质谷氨酸再循环功能障碍一致。
