Lui Jia-ming, Fu Yan, Yu Bing-bin, Li Long-di
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2002 Jun;22(3):423-6.
The properties of room temperature phosphorescence (RTP) of the labeled goat anti-human antibody(with fluorescein isothiocynate GAHAb-FITC) and labeled rabit anti-goat antibody(RAGAb-FITC) with FITC in different dilution ratios on many kinds of solid substrates were studied in detail. It is found that on the polyamide membrane, only using Pb(Ac)2 as a heavy atom perturber, both the labeled antibodies and their antigen-antibody conjugated compound with the human immunoglobulin G remained excellent RTP properties of isothiocyanate fluorescein, lambda ex(max)/lambda em(max) = 525/650 nm, the relation of RTP intensity to the concentration was linear in a certain range. The conditions for immunological reaction and RTP emission were optimized. The result means that it is possible to develop a new immunoassay named as solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA), which combines both the specificity of immunological reaction and high sensitivity of luminescence analysis.
详细研究了异硫氰酸荧光素标记的山羊抗人抗体（GAHAb-FITC）和异硫氰酸荧光素标记的兔抗山羊抗体（RAGAb-FITC）在多种固体基质上不同稀释比例的室温磷光（RTP）性质。发现在聚酰胺膜上，仅使用醋酸铅作为重原子微扰剂时，标记抗体及其与人免疫球蛋白G的抗原-抗体共轭化合物均保持异硫氰酸荧光素优异的RTP性质，激发波长最大值/发射波长最大值 = 525/650 nm，RTP强度与浓度在一定范围内呈线性关系。优化了免疫反应和RTP发射的条件。结果表明，有可能开发一种名为固体基质室温磷光免疫分析（SS-RTP-IA）的新型免疫分析方法，该方法结合了免疫反应的特异性和发光分析的高灵敏度。
