Preparation of adriamycin magnetic albumin microspheres and their experimental antitumor effects in vitro and in vivo.

The adriamycin magnetic microspheres (ADM-MAMs) were prepared by the heat-stabilized protein methods. Their physico-chemical properties were examined; their cytotoxicities against tumor cells in vitro were assayed by a modified MTT method, and their effects were observed on the implanted gastric tumor in Wistar rats given ADM-MAMs via alimentary canal at the presence of the external magnetic fields. The results showed that the ADM-MAMs were successfully prepared and had cytotoxic effect on tumor cells in vitro similar to the free ADM (P > 0.05). The inhibitory effects of ADM-MAMs on the implanted gastric tumor in vivo were significantly increased as compared with the controls (P < 0.01). Our results suggested that ADM-MAMs were a new type of adriamycin (ADM) preparation and its form alteration did not affect its anticancer effects.