[在无血清培养基上培养小鼠和人类淋巴细胞]

[The cultivation of mouse and human lymphoid cells on serum-free media].

作者信息

Nikolaenko N S, Tsupkina N V, Pinaev G P

出版信息

Tsitologiia. 1992;34(8):88-95.

Abstract

The optimum composition of several serum-free media has been established for a long-term cultivation of hybridomas, lymphoid and erythroleukemic cells. The medium DME/F12 appeared to be the medium of choice. It is necessary to supplement the basic medium with lipid and iron transport proteins (bovine serum albumin, transferrin) and peptide hormone (insulin) for obtaining stable results. However, there are differences in successful growth of examined cell lines under serum-free conditions: some of them acquire saturation density comparable with that of the control medium (hybridomas derived from myeloma Sp2/0-Ag14, cell lines K-562, Raji) but other lines do not (hybridoma derived from myeloma NS0/1, cell lines Namalwa, RPMI 1788, Molt-4). Thus, these serum-free media are not universal, therefore each new hybridoma and cell line should be tested to determine the suitability for them of some proposed media. The high effectiveness of cultivation under serum-free conditions can be presumably achieved by optimization of both qualitative and quantitative composition of the serum replacement and of the basic medium.

摘要

已经确定了几种无血清培养基的最佳组成，用于杂交瘤、淋巴细胞和红白血病细胞的长期培养。DME/F12培养基似乎是首选培养基。为了获得稳定的结果，有必要在基础培养基中补充脂质和铁转运蛋白（牛血清白蛋白、转铁蛋白）以及肽激素（胰岛素）。然而，在无血清条件下，所检测的细胞系的成功生长存在差异：其中一些细胞系达到的饱和密度与对照培养基相当（源自骨髓瘤Sp2/0-Ag14的杂交瘤、K-562细胞系、Raji细胞系），但其他细胞系则不然（源自骨髓瘤NS0/1的杂交瘤、Namalwa细胞系、RPMI 1788细胞系、Molt-4细胞系）。因此，这些无血清培养基并非通用，所以对于每种新的杂交瘤和细胞系，都应进行测试，以确定某些推荐培养基对它们的适用性。通过优化血清替代物和基础培养基的定性和定量组成，大概可以实现无血清条件下培养的高效性。