Fromm M, Hattemer H H
Institut für Forstgenetik und Forstpflanzenzüchtung, Universität Göttingen, Büsgenweg 2, D-37077 Göttingen, Germany.
Heredity (Edinb). 2003 Sep;91(3):337-44. doi: 10.1038/sj.hdy.6800325.
Inheritance analysis of seven enzyme systems in hexaploid Tilia cordata Mill. was performed utilizing single trees and their open-pollinated progenies. Genetic analyses of 12 polymorphic gene loci showed that T. cordata had disomic inheritance despite being an allopolyploid. T. platyphyllos Scop. was also used in the investigations although no genetic inheritance analysis was carried out. In comparison with T. cordata, zymograms of 10 spontaneous T. cordata x T. platyphyllos hybrids showed markedly different banding patterns with species-specific alleles at 13 of the 14 described gene loci. Hence, differentiation between both species and their naturally occurring hybrid (T. x europaea) is easily feasible with allozyme studies.
利用单株树木及其自由授粉后代对六倍体心叶椴(Tilia cordata Mill.)的七个酶系统进行了遗传分析。对12个多态性基因位点的遗传分析表明,尽管心叶椴是异源多倍体,但它具有二体遗传。虽然没有进行遗传遗传分析,但也使用了阔叶椴(T. platyphyllos Scop.)进行研究。与心叶椴相比,10个自发的心叶椴×阔叶椴杂种的酶谱显示出明显不同的条带模式,在所描述的14个基因位点中的13个位点上具有物种特异性等位基因。因此,通过同工酶研究很容易区分这两个物种及其天然杂种(欧洲椴(T. x europaea))。