Zhang Jian-Zhen, Ma En-Bo, Guo Ya-Ping
College of Life Science and Technology, Shanxi University, Taiyuan 030006, China.
Yi Chuan Xue Bao. 2003 Jun;30(6):533-9.
The molecular phylogenetic relationships of five species of Oxya Audient-serville including O. chinensis, O. brachyptera, O. agavisa, O. japonica and O. intricata were studied with RAPD. Genomic DNA of forty-one individuals were amplified with eight oligonucleotide (10 mer) primers which were previously selected, the specifical bands occured in all them, a total of 96 clear and reproducible bands (rang from 200-2500 bp) were generated, 95 of which were polymorphic band, the only one band (850 bp) which was amplified with S362 was common to five species of Oxya. The obtaining segments of individual primer were between 8-16, the average was 12. A molecular phylogenetic tree based on was constructed Euclidean distance among five species of rice grasshopper by between-groups linkage method, the result indicated O. chinensis was closely related to O. brachyptera, the genetic relationship of O. japonica and O. agavisa was also close, whereas O. intricata had far phylogenetic relationship with them. The results of dendrogram were consistent with the previous conclusion of morphologic classification and cytotaxonomy, and suggested RAPD was suitable for analysis of phylogenetic relationships among species of Oxya.
运用随机扩增多态性DNA（RAPD）技术对中华稻蝗、短翅稻蝗、无齿稻蝗、日本稻蝗和曲胫稻蝗5种稻蝗的分子系统发育关系进行了研究。用预先筛选的8个10聚体寡核苷酸引物对41个个体的基因组DNA进行扩增，所有个体均出现特异性条带，共产生96条清晰且可重复的条带（片段大小在200 - 2500 bp之间），其中95条为多态性条带，用S362引物扩增出的唯一一条850 bp的条带为5种稻蝗所共有。单个引物获得的片段数在8 - 16条之间，平均为12条。采用类平均法基于欧氏距离构建了5种稻蝗的分子系统发育树，结果表明中华稻蝗与短翅稻蝗亲缘关系较近，日本稻蝗与无齿稻蝗的亲缘关系也较近，而曲胫稻蝗与它们的亲缘关系较远。聚类图结果与以往形态分类和细胞分类学的结论一致，表明RAPD技术适用于稻蝗属物种间系统发育关系的分析。
