Klimiuk Piotr A, Sierakowski Stanisław, Chwiećko Justyna
Klinika Reumatologii i Chorób Wewnetrznych Akademii Medycznej w Białymstoku.
Pol Merkur Lekarski. 2003 May;14(83):410-2.
Tumour necrosis factor alpha (TNF-alpha) and matrix metalloproteinases (MMPs) play an important role in the pathogenesis of rheumatoid arthritis (RA). The present study was conducted to investigate whether the serum level of TNF-alpha is correlated with MMPs and tissue inhibitors of metalloproteinases (TIMPs) in RA patients. Serum concentrations of TNF-alpha, interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), gelatinase B (MMP-9), TIMP-1 and TIMP-2 were measured by ELISA in 34 patients with RA. We found the TNF-alpha to correlate with MMP-1, MMP-3, MMP-9 and total measured MMPs serum concentrations (p < 0.05 for all comparisons). Furthermore, serum TNF-alpha, MMP-1 MMP-3, MMP-9 and TIMP-1 levels correlated with markers of disease activity such as the erythrocyte sedimentation rate, C reactive protein level and the number of swollen joints. No associations were observed between TNF-alpha and TIMPs serum concentrations. Our results support the concept of the regulation of the MMPs synthesis by cytokines such as TNF-alpha. We conclude that the measurement of the serum TNF-alpha, MMPs and TIMP-1 concentrations may be useful in the assessment of RA activity.
肿瘤坏死因子α（TNF-α）和基质金属蛋白酶（MMPs）在类风湿关节炎（RA）的发病机制中起重要作用。本研究旨在调查RA患者血清TNF-α水平是否与MMPs及金属蛋白酶组织抑制剂（TIMPs）相关。采用酶联免疫吸附测定法（ELISA）检测了34例RA患者血清中TNF-α、间质胶原酶（MMP-1）、基质溶解素-1（MMP-3）、明胶酶B（MMP-9）、TIMP-1和TIMP-2的浓度。我们发现TNF-α与MMP-1、MMP-3、MMP-9及所测MMPs血清总浓度相关（所有比较p<0.05）。此外，血清TNF-α、MMP-1、MMP-3、MMP-9和TIMP-1水平与疾病活动指标如红细胞沉降率、C反应蛋白水平及肿胀关节数相关。未观察到TNF-α与TIMPs血清浓度之间存在关联。我们的结果支持细胞因子如TNF-α调节MMPs合成这一概念。我们得出结论，检测血清TNF-α、MMPs和TIMP-1浓度可能有助于评估RA的活动度。
