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迈向植物中更具通用性的α-葡聚糖生物合成。

Towards a more versatile alpha-glucan biosynthesis in plants.

作者信息

Kok-Jacon Géraldine A, Ji Qin, Vincken Jean-Paul, Visser Richard G F

机构信息

Wageningen University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.

出版信息

J Plant Physiol. 2003 Jul;160(7):765-77. doi: 10.1078/0176-1617-01028.

DOI:10.1078/0176-1617-01028
PMID:12940545
Abstract

Starch is an important storage polysaccharide in many plants. It is composed of densely packed alpha-glucans, consisting of 1,4- and 1,4,6-linked glucose residues. The starch polymers are used in many industrial applications. The biosynthetic machinery for assembling the granule has been manipulated in many different ways to gain insight into the process of starch biosynthesis and to engineer starches with improved functionalities. With respect to the latter, two generic technologies with great potential have been developed: (i) introduction of new linkage types in starch polymers (1,3- and 1,6-linkages), and (ii) engineering granule-boundness. The toolbox to engineer this new generation of starch polymers is discussed.

摘要

淀粉是许多植物中一种重要的储存多糖。它由紧密堆积的α-葡聚糖组成,这些α-葡聚糖由1,4-和1,4,6-连接的葡萄糖残基构成。淀粉聚合物被用于许多工业应用中。为了深入了解淀粉生物合成过程并设计出具有改良功能的淀粉,人们以多种不同方式对组装颗粒的生物合成机制进行了操控。关于后者,已开发出两种具有巨大潜力的通用技术:(i)在淀粉聚合物中引入新的连接类型(1,3-和1,6-连接),以及(ii)设计颗粒结合性。本文讨论了用于设计新一代淀粉聚合物的工具箱。

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引用本文的文献

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The future of starch bioengineering: GM microorganisms or GM plants?淀粉生物工程的未来:转基因微生物还是转基因植物?
Front Plant Sci. 2015 Apr 23;6:247. doi: 10.3389/fpls.2015.00247. eCollection 2015.
2
Barley callus: a model system for bioengineering of starch in cereals.大麦愈伤组织:谷物中淀粉生物工程的模式系统。
Plant Methods. 2012 Sep 7;8(1):36. doi: 10.1186/1746-4811-8-36.
3
High level accumulation of alpha-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans.通过表达变形链球菌的gtfD基因在玉米籽粒中高水平积累α-葡聚糖。
Transgenic Res. 2007 Aug;16(4):467-78. doi: 10.1007/s11248-006-9049-8. Epub 2006 Nov 25.
4
Expression of alternansucrase in potato plants.交替蔗糖酶在马铃薯植株中的表达
Biotechnol Lett. 2007 Jul;29(7):1135-42. doi: 10.1007/s10529-007-9348-z. Epub 2007 Mar 23.
5
Fusion proteins comprising the catalytic domain of mutansucrase and a starch-binding domain can alter the morphology of amylose-free potato starch granules during biosynthesis.包含变形链球菌蔗糖酶催化结构域和淀粉结合结构域的融合蛋白在生物合成过程中可改变无直链淀粉马铃薯淀粉颗粒的形态。
Transgenic Res. 2007 Oct;16(5):645-56. doi: 10.1007/s11248-006-9053-z. Epub 2006 Dec 8.
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Accumulation of multiple-repeat starch-binding domains (SBD2-SBD5) does not reduce amylose content of potato starch granules.多个重复淀粉结合结构域(SBD2-SBD5)的积累不会降低马铃薯淀粉颗粒的直链淀粉含量。
Planta. 2007 Mar;225(4):919-33. doi: 10.1007/s00425-006-0411-0.
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Production of dextran in transgenic potato plants.转基因马铃薯植株中葡聚糖的产生。
Transgenic Res. 2005 Aug;14(4):385-95. doi: 10.1007/s11248-005-0439-0.