Shults Melissa D, Pearce Dierdre A, Imperiali Barbara
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
J Am Chem Soc. 2003 Sep 3;125(35):10591-7. doi: 10.1021/ja0355980.
A modular peptide scaffold has been developed for fluorescent sensing of divalent zinc. The signaling component of the chemosensor is the chelation-sensitive fluorophore 8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline, which is prepared as the protected amino acid derivative Fmoc-Sox-OH and integrated into peptide sequences. Nineteen synthetic peptides incorporating the signaling element exhibit a range of affinities for Zn(2+) through variation of the type and number of Zn(2+) ligands, ligand arrangement and the beta-turn sequence that acts as a preorganization element between the ligands. The stoichiometry of the peptide-Zn(2+) complexes is evaluated by several criteria. The fluorescence response of these peptides to pH and various important metal ions is reported. Eleven of these sequences form only 1:1 complexes with Zn(2+) and their affinities range from 10 nM to nearly 1 microM. When used in concert, these sensors can provide Zn(2+) concentration information in a valuable range.
一种用于二价锌荧光传感的模块化肽支架已被开发出来。化学传感器的信号传导成分是对螯合敏感的荧光团8-羟基-5-(N,N-二甲基磺酰胺基)-2-甲基喹啉，它被制备为受保护的氨基酸衍生物Fmoc-Sox-OH并整合到肽序列中。通过改变锌离子配体的类型和数量、配体排列以及作为配体之间预组织元件的β-转角序列，19种包含信号元件的合成肽对Zn(2+)表现出一系列亲和力。通过几个标准评估肽-Zn(2+)配合物的化学计量。报道了这些肽对pH值和各种重要金属离子的荧光响应。这些序列中的11个仅与Zn(2+)形成1:1配合物，其亲和力范围从10 nM到近1 microM。当协同使用时，这些传感器可以在有价值的范围内提供Zn(2+)浓度信息。
