Moon Sang-Kwan, Kim Young-Suk, Ko Chang-Nam, Bae Hyung-Sup, Cho Ki-Ho, Lee Kyung-Sup
Department of Circulatory Internal Medicine, College of Oriental Medicine, Kyung-Hee University, Seoul, Korea.
Am J Chin Med. 2003;31(3):389-402. doi: 10.1142/S0192415X03001168.
For molecular biological characterization of the effects of Uwhangchungsimwon (UC) on the expression of nitric oxide synthase (NOS) gene and cell adhesion-regulating gene, vascular cell adhesion molecule-1 (VCAM-1), the human endothelial cell line (ECV304) was treated with the extract of UC and transcription of genes was examined using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. UC showed a transcription-activating effect on the NOS gene and a suppressing effect on the VCAM-1 gene in human endothelial cells, and these effects were found in a dose- and time-dependent manner. Down-regulation of VCAM-1 expression by UC was directly mediated by increased nitric oxide (NO) production, which was associated with increased NOS gene transcription. This study strongly suggests that the clinical effects of UC on stroke might be derived at least in part from its ability to induce NOS expression, which was followed by significant reduction of VCAM-1 expression.
为了从分子生物学角度表征牛黄清心丸（UC）对一氧化氮合酶（NOS）基因和细胞黏附调节基因血管细胞黏附分子-1（VCAM-1）表达的影响，用人内皮细胞系（ECV304）进行牛黄清心丸提取物处理，并采用定量逆转录聚合酶链反应（RT-PCR）分析来检测基因转录情况。牛黄清心丸对人内皮细胞中的NOS基因具有转录激活作用，对VCAM-1基因具有抑制作用，且这些作用呈现剂量和时间依赖性。牛黄清心丸对VCAM-1表达的下调是由一氧化氮（NO）生成增加直接介导的，这与NOS基因转录增加相关。本研究有力地表明，牛黄清心丸对中风的临床疗效可能至少部分源于其诱导NOS表达的能力，随后VCAM-1表达显著降低。
