使用锁核酸（LNA）进行荧光原位杂交（FISH）：不同LNA/DNA混合寡聚核苷酸的评估
FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers.
作者信息
Silahtaroglu Asli N, Tommerup Niels, Vissing Henrik
机构信息
Wilhelm Johannsen Centre for Functional Genome Research, Department of Medical Genetics, IMBG, The Panum Institute, University of Copenhagen, Building 24.4, Blegdamsvej 3, 2200, Copenhagen N, Denmark.
出版信息
Mol Cell Probes. 2003 Aug;17(4):165-9. doi: 10.1016/s0890-8508(03)00048-3.
Locked Nucleic Acids (LNA) constitute a novel class of DNA analogues that have an exceptionally high affinity towards complementary DNA and RNA. Using human classical satellite-2 repeat sequence clusters as targets, we demonstrate that LNA/DNA mixmers oligonucleotides are excellent probes for FISH combining high binding affinity with short hybridization time and even with the ability to hybridize without prior thermal denaturation of the template.
锁核酸（LNA）是一类新型的DNA类似物，对互补DNA和RNA具有极高的亲和力。以人类经典卫星2重复序列簇为靶点，我们证明了LNA/DNA混合mers寡核苷酸是用于荧光原位杂交（FISH）的优秀探针，它结合了高结合亲和力、短杂交时间，甚至无需对模板进行热变性就能杂交的能力。
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