Elleder M
Histochemistry. 1976 Feb 16;46(2):161-5. doi: 10.1007/BF02462740.
A simple and efficient method for the demonstration of highly water soluble acid mucosubstances in cold microtone sections is described. It consists of prolonged treatment of cold microtome sections with methanol (for at least 1 h) and subsequent staining with 0.1% azure A in distilled water or in 30% methanol. The procedure is recommended particularly for the bioptical examination of mucopolysaccharidoses.
本文描述了一种在冷冻切片中显示高水溶性酸性粘多糖物质的简单有效方法。该方法包括用甲醇对冷冻切片进行长时间处理（至少1小时），随后用0.1%的天青A在蒸馏水或30%甲醇中进行染色。该方法特别推荐用于粘多糖贮积症的活检检查。
