Reconstitution of denatured E. coli alkaline phosphatase with E. coli ribosome.
作者信息
Das B, Gupta C D
机构信息
Department of Biophysics, Molecular Biology and Genetics, University of Calcutta.
出版信息
Indian J Biochem Biophys. 1992 Dec;29(6):512-5.
PMID:1294471
Abstract
E. coli alkaline phosphatase was denatured by physical/chemical means. In vitro reconstitution of this denatured enzyme was assisted by 70S E. coli ribosome, as shown by the recovery of its catalytic competence. Almost total recovery of activity of the totally inactivated enzyme was obtained in presence of equimolar concentration of 70S ribosome at 50 degrees C.
摘要
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