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[丁香假单胞菌致病变种PV.萎缩芽孢杆菌脂多糖-蛋白质复合物对根癌土壤杆菌诱导肿瘤形成过程的影响]

[Effect of the lipopolysaccharide-protein complex of Pseudomonas syringae PV. atrofaciens on the process of tumor formation caused by Agrobacterium tumefaciens].

作者信息

Hvozdiak R I, Pasichnyk L A, Vashchenko L M

机构信息

Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.

出版信息

Mikrobiol Z. 2003 May-Jun;65(3):5-13.

Abstract

It has been found that the agent of bacterial spotting of rye P. syringae pv. atrofaciens 8281 has no antagonistic action on the strains 9052 and 9054 of Agrobacterium tumefaciens--the agent of crown gall tumor. Lipopolysaccharide-protein complex of P. syringae pv. atrofaciens 8281 when added to the culture medium does not affect the growth and development of A. tumefaciens bacteria. It has been experimentally established that the lipopolysaccharide-protein complex of P. syringae pv. atrofaciens 8281, when used to treat the potato explants before inoculation of A. tumefaciens, decreases induction and development of tumours on the explants. Tumour formation inhibition depends on concentration of lipopolysaccharide-protein complex solution.

摘要

已发现,黑麦细菌性斑点病菌丁香假单胞菌致萎缩病变种8281对根癌土壤杆菌(冠瘿瘤病菌)的9052和9054菌株没有拮抗作用。当将丁香假单胞菌致萎缩病变种8281的脂多糖-蛋白质复合物添加到培养基中时,不会影响根癌土壤杆菌的生长和发育。通过实验确定,在接种根癌土壤杆菌之前,用丁香假单胞菌致萎缩病变种8281的脂多糖-蛋白质复合物处理马铃薯外植体,可减少外植体上肿瘤的诱导和发育。肿瘤形成抑制取决于脂多糖-蛋白质复合物溶液的浓度。

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