Pasquié J L, Biousse N, Mimran A, Jover B
Groupe Rein et Hypertension, Institut universitaire de recherche clinique, Université Montpellier I, rue de la Cardonille, 34293 Montpellier.
Arch Mal Coeur Vaiss. 2003 Jul-Aug;96(7-8):800-3.
The regulation of angiogenesis involves complex interactions. The aim of our study was to assess the influence of angiotensin II (ANG II) on different vascular beds in rat. Aortic, renal and mesenteric rings from 10 male Sprague-Dawley rats were cultured using a three-dimensional culture system consisting of rat type I collagen lattice. We assessed the influence of different ANG II concentrations (10(-7) et 10(-9) mol/L) on these rings as well as the effect of AT1 blockade by losartan (10(-7 mol/L). ANG II inhibited angiogenesis at 10(-7) mol/L on renal artery. However, these was a angiogenic effect at 10(-9) mol/L on the mesenteric artery. Every time losartan prevented the effect of ANG II in any kind of vessel rings. No significant effect on ANG II was found on aortic rings but coadministration of losartan induced a dramatic decrease in the number of capillary sprouts. In conclusion, ANG II seems to be deeply involved in angiogenesis. However, its effect depends on the concentration of ANG II and the type of vessel. ANG II appears to be angiogenic on mesenteric arteries via an AT1 receptor effect and mostly anti-angiogenic on the renal arteries with possible involvement of AT2 receptors.
血管生成的调节涉及复杂的相互作用。我们研究的目的是评估血管紧张素II（ANG II）对大鼠不同血管床的影响。使用由大鼠I型胶原晶格组成的三维培养系统，培养10只雄性Sprague-Dawley大鼠的主动脉、肾和肠系膜环。我们评估了不同浓度的ANG II（10^(-7)和10^(-9)mol/L）对这些环的影响以及氯沙坦（10^(-7)mol/L）对AT1的阻断作用。ANG II在10^(-7)mol/L时抑制肾动脉的血管生成。然而，在10^(-9)mol/L时对肠系膜动脉有促血管生成作用。每次氯沙坦都能阻止ANG II在任何类型血管环中的作用。未发现ANG II对主动脉环有显著影响，但氯沙坦的共同给药导致毛细血管芽数量急剧减少。总之，ANG II似乎深度参与血管生成。然而，其作用取决于ANG II的浓度和血管类型。ANG II似乎通过AT1受体效应在肠系膜动脉上具有促血管生成作用，而在肾动脉上主要具有抗血管生成作用，可能涉及AT2受体。
