Fan Z F, Du L M, Ji X, Xie H M
Center of Analysis and Testing, Shanxi Teacher's University, LinFen 041004, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Oct;21(5):682-4.
Rare earth metal ions and tryptophan form ion-association complex in basic medium. The complex causes fluorescence quenching of tryptophan. Fluorescence emission of tryptophan and quenching caused by rare earth metal ions both reach a climax in H3BO4-HAc-H3PO4-NaOH at pH 10 to 11, and all rare earth metal ions have the approximate effects on fluorescence quenching of tryptophan. The molecular mode of complex of rare earth metal ions and tryptophan has been founded and mechanism of fluorescence quenching has been studied in this paper.
稀土金属离子与色氨酸在碱性介质中形成离子缔合物。该缔合物导致色氨酸的荧光猝灭。色氨酸的荧光发射以及由稀土金属离子引起的猝灭在pH为10至11的H3BO4-HAc-H3PO4-NaOH体系中均达到最大值，并且所有稀土金属离子对色氨酸荧光猝灭的影响大致相同。本文建立了稀土金属离子与色氨酸缔合物的分子模型并研究了荧光猝灭机理。
