Marcano Ana Karina, Molina Guevara Pedro, Oropeza Maira, de García Eva
Laboratorio de Biotecnología Vegetal, Instituto de Biología Experimental (IBE), Facultad de Ciencias, Universidad Central de Venezuela, Apartado postal 47114 Los Chaguaramos, Caracas 1041, Venezuela.
Acta Cient Venez. 2002;53(4):251-7.
Efficient embryogenic callus formation (70%) in many sugarcane cultivars, has been established using young leaf explants cultivated on modified Murashige and Skoog medium containing 13 microM 2,4-dichlorophenoxiacetic acid (2,4-D). However, Venezuelan sugarcane cultivars V78-1 and V75-6 produced only 30% of embryogenic callus when were cultured in these conditions. In order to improve somatic embryogenesis in these Venezuelan cultivars, embryogenic calli were induced using different media: C3 (13 microM 2,4-D); C7 (31.5 microM 2,4-D); Cd (30 microM Dicamba) and C5BA (22.5 microM 2,4-D, 22.2 microM N6-Benzyladenine). After 45 days of induction, the highest embryogenic callus production (90%), was observed in both cultivars, when they were cultured on Cd medium. Moreover, plant development from somatic embryos originated in this callus, was evident four days after incubation on regeneration medium (without hormones), while the somatic embryos originated from calli growing in C3, C7 and C5BA media, gave rise to plants eight days after incubation on regeneration medium.
利用在含有13微摩尔2,4 - 二氯苯氧乙酸（2,4 - D）的改良Murashige和Skoog培养基上培养的幼叶外植体，已在许多甘蔗品种中建立了高效的胚性愈伤组织形成（70%）。然而，委内瑞拉甘蔗品种V78 - 1和V75 - 6在这些条件下培养时，仅产生30%的胚性愈伤组织。为了提高这些委内瑞拉品种的体细胞胚胎发生，使用不同的培养基诱导胚性愈伤组织：C3（13微摩尔2,4 - D）；C7（31.5微摩尔2,4 - D）；Cd（30微摩尔麦草畏）和C5BA（22.5微摩尔2,4 - D，22.2微摩尔N6 - 苄基腺嘌呤）。诱导45天后，当在Cd培养基上培养时，在这两个品种中均观察到最高的胚性愈伤组织产量（90%）。此外，在再生培养基（无激素）上培养4天后，起源于这种愈伤组织的体细胞胚胎的植株发育明显，而起源于在C3、C7和C5BA培养基上生长的愈伤组织的体细胞胚胎，在再生培养基上培养8天后形成植株。
