Kiyofuji Masa-aki, Iyama Ken-ichi, Kitaoka Mitsuhiko, Sado Yoshikazu, Ninomiya Yoshifumi, Ueda Shou-ichi
Department of Surgical Pathology, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Histochem J. 2002 Oct;34(10):479-86. doi: 10.1023/a:1024753705556.
Type IV collagen is a major component of the basement membrane (BM), which consists of six genetically distinct alpha(IV) chains. In this study the expression of these six alpha(IV) chains was demonstrated immunohistochemically. In addition, the alpha2(IV) and alpha5(IV) chains were analysed quantitatively by confocal laser scanning microscopy in human urogenital epithelial BM. The alpha1/alpha2(IV) and alpha5/alpha6(IV) chains were immunoreactive in the epithelial BM, whereas, alpha3/alpha4(IV) chains were not. The quantitative analysis revealed that the amount of alpha2(IV) and alpha5(IV) chains differed in each urogenital epithelial BM. The content of alpha5(IV) chains in the epithelial BM of the bladder was differentially high, and that of the foreskin was differentially low. It is concluded that the elasticity of epithelial BM of the bladder may be structurally related to the high content of alpha5/alpha6(IV) chains.
IV型胶原是基底膜（BM）的主要成分，基底膜由六种基因不同的α（IV）链组成。在本研究中，通过免疫组织化学方法证实了这六种α（IV）链的表达。此外，利用共聚焦激光扫描显微镜对人泌尿生殖上皮基底膜中的α2（IV）和α5（IV）链进行了定量分析。α1/α2（IV）和α5/α6（IV）链在上皮基底膜中具有免疫反应性，而α3/α4（IV）链则没有。定量分析表明，每种泌尿生殖上皮基底膜中α2（IV）和α5（IV）链的含量不同。膀胱上皮基底膜中α5（IV）链的含量差异较高，而包皮中的含量差异较低。得出的结论是，膀胱上皮基底膜的弹性可能在结构上与α5/α6（IV）链的高含量有关。
