Kebebew Electron, Treseler Patrick A, Duh Quan-Yang, Clark Orlo H
Department of Surgery/Department of Pathology, University of California, San Francisco, CA 94143, USA.
Surgery. 2003 Aug;134(2):235-41. doi: 10.1067/msy.2003.227.
Members of the Id helix-loop-helix proteins are key regulators of cell growth and differentiation in a variety of cell types. They are also required for cell cycle progression and regulate tumor angiogenesis. Furthermore, deregulated expression of Id proteins has been observed in some human malignancies. Therefore we hypothesized that the Id-1 gene may play a role in papillary thyroid carcinogenesis.
Id-1 gene expression was characterized by Northern blot analysis and Id-1 immunohistochemistry in human thyroid tissue. Id-1 gene expression and regulation was evaluated in a human papillary thyroid cancer cell line, TPC-1, by Northern blot analysis.
The Id-1 gene was significantly overexpressed in papillary thyroid cancer compared with normal thyroid tissue from the same patients (n = 18) by both Northern blot analysis and semiquantitative Id-1 immunohistochemistry (P <.001). Id-1 immunoreactivity was primarily localized to the cytoplasm of the thyroid follicular cells. In the TPC-1 cell line, stimulation by TSH and serum up-regulated Id-1 mRNA expression 1.5- and 4.0-fold, respectively. Activation of the mitogen intracellular protein kinase A and protein kinase C signaling pathways also up-regulated Id-1 mRNA expression. Inhibition of Id-1 mRNA expression with Id-1 antisense oligonucleotide inhibited growth compared with control Id-1 sense and random oligonucleotides (P <.05).
The Id-1 gene is overexpressed in papillary thyroid cancer. Id-1 may play a role in the regulation of growth in papillary thyroid cancer.
Id螺旋-环-螺旋蛋白家族成员是多种细胞类型中细胞生长和分化的关键调节因子。它们也是细胞周期进程所必需的，并调节肿瘤血管生成。此外，在一些人类恶性肿瘤中已观察到Id蛋白表达失调。因此，我们推测Id-1基因可能在甲状腺乳头状癌的发生中起作用。
通过Northern印迹分析和Id-1免疫组织化学对人甲状腺组织中的Id-1基因表达进行表征。通过Northern印迹分析评估人甲状腺乳头状癌细胞系TPC-1中Id-1基因的表达和调控。
通过Northern印迹分析和半定量Id-1免疫组织化学，与来自同一患者（n = 18）的正常甲状腺组织相比，Id-1基因在甲状腺乳头状癌中显著过表达（P <.001）。Id-1免疫反应性主要定位于甲状腺滤泡细胞的细胞质中。在TPC-1细胞系中，促甲状腺激素（TSH）和血清刺激分别使Id-1 mRNA表达上调1.5倍和4.0倍。丝裂原细胞内蛋白激酶A和蛋白激酶C信号通路的激活也上调了Id-1 mRNA表达。与对照Id-1正义寡核苷酸和随机寡核苷酸相比，用Id-1反义寡核苷酸抑制Id-1 mRNA表达可抑制生长（P <.05）。
Id-1基因在甲状腺乳头状癌中过表达。Id-1可能在甲状腺乳头状癌的生长调节中起作用。
