Ramos Eduardo J B, Xu Yuan, Romanova Irina, Middleton Frank, Chen Chung, Quinn Robert, Inui Akio, Das Undurti, Meguid Michael M
Department of Surgery, Management Information and Decision, Clinical Molecular Medicine, SUNY Upstate Medical University, Syracuse University, Syracuse, NY, USA.
Surgery. 2003 Aug;134(2):329-35. doi: 10.1067/msy.2003.267.
Most obese individuals have elevated concentrations of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), markers of inflammation closely associated with diabetes, hypertension, and stroke.
Obesity is a low-grade inflammatory disease, and Roux-en-Y gastric bypass (RYGB) reduces biochemical markers of inflammation and modifies gene expression in hypothalamic food intake/energy-related nuclei and subcutaneous abdominal fat (SAF).
Obesity was induced in 24 3-week-old Sprague Dawley pups fed a high-energy diet (HED). Three groups (n = 8/group) were studied: RYGB, sham-operated pair-fed, and sham-operated ad libitum HED. Controls were nonobese rats fed chow (n = 6). Rats were killed 10 days after operation, and blood was collected to measure corticosterone and SAF and mesenteric fat to measure IL-6, TNF-alpha, and corticosterone. Total mRNA from arcuate nucleus and SAF purified for gene expression profiling. Data were analyzed with analysis of variance, Mann-Whitney test, and t test.
Before operation, the body weight of the obese groups was 493 +/- 7 g and control = 394 +/- 12g. The 10-day postoperative weight was RYGB = 417 +/- 21 g, pair-fed = 436 +/- 14 g, and ad libitum HED = 484 +/- 15 g. Mesenteric and SAF weight decreased in RYGB. Mesenteric/SAF ratio of IL-6, TNF-alpha, corticosterone, and gene profiling showed decrease of inflammation after RYGB.
Gastric bypass reduces biochemical markers of inflammation, suggesting that obesity is an inflammatory condition.
大多数肥胖个体的白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）浓度升高，这些炎症标志物与糖尿病、高血压和中风密切相关。
肥胖是一种低度炎症性疾病， Roux-en-Y胃旁路术（RYGB）可降低炎症的生化标志物，并改变下丘脑食物摄入/能量相关核团及腹部皮下脂肪（SAF）中的基因表达。
给24只3周龄的斯普拉格-道利幼鼠喂食高能饮食（HED）以诱导肥胖。研究了三组（每组n = 8）：RYGB组、假手术配对喂养组和假手术自由摄食HED组。对照组为喂食普通饲料的非肥胖大鼠（n = 6）。术后10天处死大鼠，采集血液测定皮质酮，采集SAF和肠系膜脂肪测定IL-6、TNF-α和皮质酮。纯化弓状核和SAF的总mRNA用于基因表达谱分析。数据采用方差分析、曼-惠特尼检验和t检验进行分析。
术前，肥胖组体重为493±7 g，对照组为394±12 g。术后10天体重分别为：RYGB组 = 417±21 g，配对喂养组 = 436±14 g，自由摄食HED组 = 484±15 g。RYGB组的肠系膜和SAF重量减轻。RYGB术后，肠系膜/SAF中IL-6、TNF-α、皮质酮的比例及基因谱显示炎症减轻。
胃旁路术可降低炎症的生化标志物，提示肥胖是一种炎症状态。
