Chen B, Meng F, Ma H, Ding Y, Jin L, Chen D
Molecular and Nano-Sciences Laboratory, Department of Physics, Tsinghua University, 100084 Beijing.
Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Jun;21(3):263-6.
Fluorescence correlation spectroscopy (FCS) is a powerful tool for probing biological process inside living cells. It measures fluorescence fluctuations of small number of molecules and derive information on molecular kinetics and reactions. We have developed a Monte Carlo model to simulate Browning motion of Rayleigh particles in a laser gradient field. The simulation reveals relations between laser field strength and measured parameters from FCS, such as diffusion coefficient and number density of the particles. The simulated results agree qualitatively to the experimental results obtained using fluorescent spheres. Empirical relations from the simulation are also discussed.
荧光相关光谱法（FCS）是探测活细胞内生物过程的强大工具。它测量少量分子的荧光涨落，并获取有关分子动力学和反应的信息。我们开发了一个蒙特卡罗模型来模拟瑞利粒子在激光梯度场中的布朗运动。该模拟揭示了激光场强度与FCS测量参数之间的关系，如粒子的扩散系数和数密度。模拟结果与使用荧光球获得的实验结果在定性上一致。还讨论了模拟得出的经验关系。
