[L1210细胞株及其克隆细胞的mRNA表达与十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）]
[The expression of mRNA and SDS-PAGE of L1210 cell strains and its cloned cells].
作者信息
Liu Qiuyan, Lu Zhanjun, Wang Xiufang, Liu Fuying
机构信息
Department of Laboratory Animal, Hebei Medical University, Shijiazhuang 050017, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):207-9.
OBJECTIVE
To examine the expression difference of mRNA of L1210 cell strains and its cloned cells and discuss the methods for quality control of cell strains.
METHODS
We used SDS-PAGE to observe the difference of protein and performed in situ hybridization to examine the expression of mRNA with the use of 6 cDNA probes that were marked by biotin.
RESULTS
The number of protein bands of L1210 from Beijing Cancer Institute was 32. The number of protein bands of the two cloned cells L3E11 and L3F9 was 31. The 6 cDNA probes (p16, c-fos, c-jun, c-myc, p21, and p53 mRNA) were found to be existing in Beijing Cancer Institute L1210 and two different cloned cell strains. Expression of c-myc, c-fos, p53 mRNA could distinguish L3E11 and L3F9 cloned cells.
目的
检测L1210细胞株及其克隆细胞mRNA的表达差异，探讨细胞株质量控制方法。
方法
采用SDS-PAGE观察蛋白质差异，运用生物素标记的6种cDNA探针进行原位杂交检测mRNA表达。
结果
北京肿瘤研究所的L1210细胞蛋白质条带数为32条。两个克隆细胞L3E11和L3F9的蛋白质条带数为31条。发现6种cDNA探针（p16、c-fos、c-jun、c-myc、p21和p53 mRNA）存在于北京肿瘤研究所L1210及两种不同的克隆细胞株中。c-myc、c-fos、p53 mRNA的表达可区分L3E11和L3F9克隆细胞。
Zotero 插件