[从人宫颈黏液中分离纯化一种抗菌多肽]
[Isolation and purification of an antibacterial polypeptide from human cervical mucus].
作者信息
Yang Huarong, Pan Xiaoling, Huang Ning, Wu Qi, Feng Yun, Wang Boyao
机构信息
Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):214-6.
OBJECTIVE
This study was made to isolate and purify antibacterial polypeptides from human cervical mucus.
METHODS
Human cervical mucus was collected from human healthy subjects, and the acid-soluble extract was obtained by solving the mucus with 5% acetic acid in the presence of protease inhibitor. By using gel overlay technique, one protein band that was potently antibacterial against E. coli ML-35P was identified. To purify the antibacterial polypeptide, the preparative acid-urea gel electrophoresis and Reverse Phase HPLC were performed.
RESULTS
A purified antibacterial polypeptide was obtained and named HCP-1. Tricine-SDS-PAGE indicated that the relative molecular mass of HCP-1 was around 14 x 10(3). Agar radial diffusion assay showed that the purified polypeptide had antibacterial activities against E. coli ML-35P and Pseudomanas aeruginosa ATCC65922.
CONCLUSION
HCP-1 may be a new effector molecule of human cervical mucus.
目的
本研究旨在从人宫颈黏液中分离纯化抗菌多肽。
方法
从健康受试者收集人宫颈黏液，在蛋白酶抑制剂存在的情况下用5%乙酸溶解黏液获得酸溶性提取物。通过凝胶覆盖技术，鉴定出一条对大肠杆菌ML-35P有强效抗菌作用的蛋白条带。为纯化抗菌多肽，进行了制备性酸脲凝胶电泳和反相高效液相色谱。
结果
获得一种纯化的抗菌多肽并命名为HCP-1。Tricine-SDS-PAGE表明HCP-1的相对分子质量约为14×10³。琼脂扩散法显示纯化的多肽对大肠杆菌ML-35P和铜绿假单胞菌ATCC65922有抗菌活性。
结论
HCP-1可能是人宫颈黏液的一种新的效应分子。
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