[曲安奈德对青蛙缝匠肌终板膜上乙酰胆碱受体反应性的影响]

[The effect of TWP on the reactivity of AChR on the end-plate membrane of frog sartorius].

作者信息

Li Keyong, Lü Guangneng, Zhang Yun, Wang Tao, Xu Jianguo

机构信息

Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):286-8.

PMID:12947715

Abstract

OBJECTIVE

To study the effect of Tripterygium Wilfordii Polyglycosidium (TWP) on the reactivity of n-Acetylcholine receptor (nAChR) on the end plate membrane.

METHODS

Using the microionophoretic method, we applied the exogenous acetylcholine chloride (AChCl) to the superficial end-plates of the isolated nonuniform stretched frog sartorius, and acetylcholine potential (AChP) recorded was taken as an indicator of the reactivity of the nAChR in the end plate.

RESULTS

After the addition of TWP, the apparent change of AChP was recorded at 20 min in the 20 mg/L group, and at 10 min in the 40 mg/L and 60 mg/L groups respectively. 2. Within the 20 mg/L and 40 mg/L TWP groups, the change in amplitude of AChP showed significant difference with the time (P < 0.05), the greatest being at 60 min. 3. In the 60 mg/L TWP group, action potential was recorded at 40 min, 60 min and 80 min. 4. There was significant difference in the percentage of AChP amplitude change between the three concentration groups (P < 0.05), and the highest percentage of change appeared in the 60 mg/L group.

CONCLUSION

TWP can increase the reactivity of nAChR in the end plate membrane, and there exist an apparent concentration-effect relationship and a certain time-effect relationship.

摘要

目的

研究雷公藤多苷（TWP）对终板膜上N-乙酰胆碱受体（nAChR）反应性的影响。

方法

采用微离子电泳法，将外源性氯化乙酰胆碱（AChCl）施加于分离的非均匀拉伸的蛙缝匠肌的浅终板，记录的乙酰胆碱电位（AChP）作为终板中nAChR反应性的指标。

结果

添加TWP后，20 mg/L组在20分钟时记录到AChP有明显变化，40 mg/L组和60 mg/L组分别在10分钟时记录到明显变化。2. 在20 mg/L和40 mg/L TWP组内，AChP幅度变化与时间有显著差异（P<0.05），最大变化出现在60分钟时。3. 在60 mg/L TWP组，在40分钟、60分钟和80分钟时记录到动作电位。4. 三个浓度组之间AChP幅度变化百分比有显著差异（P<0.05），变化百分比最高出现在60 mg/L组。