Watanabe A
Department of Medicine, Juntendo University School of Medicine.
Nihon Jinzo Gakkai Shi. 1992 Nov;34(11):1219-25.
The production of hydrogen peroxide (H2O2) by neutrophilic polymorphonuclear leukocytes (PMN) after stimulation with PMA, FMLP, aggregated IgG and phagocytosis were determined in 36 patients with non-insulin dependent diabetes mellitus (NIDDM). The H2O2 production of PMN after the stimulation was measured using by flow cytometry. The patients were divided into four stages as follows: (1) non-microalbuminuric stage, (2) microalbuminuric stage, (3) proteinuric stage without impairment of renal function (less than 1.2 mg/dl of serum creatinine) and (4) proteinuric stage with impairment of renal function (more than 1.3mg/dl of serum creatinine). The H2O2 production after stimulation with PMA or phagocytosis was significantly higher in patients with NIDDM than normal controls. And also, there is the tendency of an increase in the H2O2 production after stimulation with FMLP or aggregated IgG. This increase of the H2O2 production was observed in all four stages of NIDDM patients after the stimulation, especially in patients with renal failure associated with diabetic nephropathy. These results suggest that reactive oxygen species produced by PMN after stimulation under various conditions may play an important role in the progression and exacerbation of diabetic nephropathy.
在36例非胰岛素依赖型糖尿病（NIDDM）患者中，测定了用佛波酯（PMA）、N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）、聚集免疫球蛋白G（IgG）刺激以及吞噬作用后，嗜中性多形核白细胞（PMN）产生过氧化氢（H2O2）的情况。使用流式细胞术测量刺激后PMN产生H2O2的量。患者分为以下四个阶段：（1）非微量白蛋白尿阶段，（2）微量白蛋白尿阶段，（3）无肾功能损害的蛋白尿阶段（血清肌酐低于1.2mg/dl）和（4）有肾功能损害的蛋白尿阶段（血清肌酐高于1.3mg/dl）。NIDDM患者经PMA刺激或吞噬作用后产生的H2O2明显高于正常对照组。而且，经FMLP或聚集IgG刺激后，H2O2产生量也有增加的趋势。在NIDDM患者刺激后的所有四个阶段均观察到H2O2产生量的增加，尤其是在伴有糖尿病肾病的肾衰竭患者中。这些结果表明，在各种条件下刺激后PMN产生的活性氧可能在糖尿病肾病的进展和加重中起重要作用。
