Warner F D
J Cell Sci. 1976 Jan;20(1):101-14. doi: 10.1242/jcs.20.1.101.
Electron micrographs of both negatively contrasted and thin-sectioned lamellibranch gill cilia reveal several new features of ciliary fine structure, particularly in regard to those structures forming intermittent or permanent crossbridges between microtubules. Negative-contrasting reveals the presence of a 14-5-nm repeating bridge between the central microtubules. Frontal views of negatively contrasted dynein arm rows along subfibre A show that the arms (23-nm repeat) in the outer row are displaced in a left-handed manner by 3-4nm with respect to those in the inner row. This displacement is probably a direct reflexion of the helical tubulin subunit lattice of the subfibre. Interdoublet (nexin) links are seen connecting adjacent A and B subfibres at intervals of 86 nm along the doublet. Negative-contrasting shows thin, highly elastic connexions holding the doublets together. When seen in longitudinal thin sections, the interdoublet links are often tilted to considerable angles, indicating they may have an elastic response to interdoublet sliding.
对双壳类鳃纤毛进行负染和超薄切片后的电子显微镜图像揭示了纤毛精细结构的几个新特征，特别是关于那些在微管之间形成间歇性或永久性交叉桥的结构。负染显示中央微管之间存在14 - 5纳米重复桥。沿亚纤维A的负染动力蛋白臂排的正面视图表明，外排的臂（23纳米重复）相对于内排的臂以左手方式位移3 - 4纳米。这种位移可能是亚纤维微管蛋白亚基螺旋晶格的直接反映。可见双联体间（连接蛋白）连接沿双联体以86纳米的间隔连接相邻的A和B亚纤维。负染显示有细的、高弹性的连接将双联体连接在一起。在纵向超薄切片中观察时，双联体间连接常常倾斜到相当大的角度，表明它们可能对双联体滑动有弹性反应。
